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AB313943

Hamster CHO Clusterin ELISA Kit

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Hamster CHO Clusterin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Hamster CHO Clusterin in Chinese hamster in Cell Culture Media samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Culture Media

Reacts with

Chinese hamster

Assay type

Sandwich (quantitative)

Sensitivity

= 0.094 ng/mL

Range

0.3125 - 20 ng/mL

Assay time

2h 50m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Hamster CHO Clusterin ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Clusterin in Hamster (CHO) biological samples.
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.
Biological function summary

Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.

Pathways

Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.

Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.
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Product protocols

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Target data

See full target information Hamster (CHO) Clusterin
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websiteProtocolBooklet
en
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Complementary Products

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Secondary Antibodies

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