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AB313941

Hamster CHO Nidogen-1 ELISA Kit

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Hamster CHO Nidogen-1 ELISA Kit is a Sandwich ELISA for the measurement of Hamster CHO Nidogen-1 in Chinese hamster in Cell Culture Media samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Culture Media

Reacts with

Chinese hamster

Assay type

Sandwich

Results type

Quantitative

Range

0.3125 - 20 ng/mL

Assay time

2h 50m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB254325

Anti-Entactin/NID antibody [EPR22414-125]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)

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Proteins & Peptides

AB225643

Recombinant Mouse Entactin/NID protein

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Proteins & Peptides

AB276426

Recombinant Human TEM7 protein (His tag)

SDS-PAGE - Recombinant Human TEM7 protein (His tag) (AB276426)

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Proteins & Peptides

AB193473

Recombinant Hepatitis B virus Hepatitis B Surface Antigen AD protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Hamster CHO Nidogen-1 ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring NDN in Hamster (CHO) biological samples.
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313941-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB313941-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB313941-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB313941-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB313941-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated Nidogen-1 Detection Antibody 100X", "number":"AB313941-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Nidogen-1 Calibrator", "number":"AB313941-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB313941-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Nidogen-1 Antibody-coated ELISA Micro Plate", "number":"AB313941-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.
Biological function summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Pathways

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.
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Product protocols

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Target data

See full target information Hamster (CHO) Nidogen-1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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