Hamster (CHO) PLBL2 ELISA Kit

Product details

Host cell protein (HCP) impurities remain a critical quality concern in the manufacturing of monoclonal antibodies (mAbs) produced in CHO cells. Among these, phospholipase B-like 2 (PLBL2) is of particular interest due to its immunogenic potential and historical association with polysorbate degradation and formulation instability, even at trace levels. Effective monitoring and removal of PLBL2 is essential for biopharmaceutical safety and stability. CHO PLBL2 ELISA Kit provides researchers and process developers with a critical tool for monitoring and controlling PLBL2, a known high-risk HCP.

How the assay works:

In this assay the PLBL2 present in samples reacts with the anti-PLBL2 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-PLBL2, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PLBL2 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PLBL2 in the test sample. The quantity of PLBL2 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.