His-tag ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of His-tag in Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Urine, Cell culture supernatant, Tissue Extracts, Cell Extract samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Urine, Cell culture supernatant, Tissue Extracts, Cell Extract
- Assay type
Competitive
- Range
78.125 - 5000 ng/mL
- Assay time
1h 30m
- Sensitivity
= 36.9 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Targets
10X His tag®, 8X His tag®, 11X His tag®
What's included?
His-tag ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of His-tag in Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Urine, Cell culture supernatant, Tissue Extracts, Cell Extract samples.
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Urine, Cell culture supernatant, Tissue Extracts, Cell Extract
- Assay type
Competitive
- Range
78.125 - 5000 ng/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 36.9 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 5.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 4.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 99 - 109 % |
Sample type Citrate plasma | Average % = 103 | Range 95 - 108 % |
Sample type EDTA Plasma | Average % = 110 | Range 106 - 114 % |
Sample type Heparin Plasma | Average % = 102 | Range 98 - 106 % |
Sample type Urine | Average % = 98 | Range 95 - 104 % |
Sample type Cell culture supernatant | Average % = 95 | Range 92 - 96 % |
Sample type Cell Extract | Average % = 97 | Range 94 - 101 % |
Sample type Tissue Extracts | Average % = 100 | Range 99 - 102 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
His-tag SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of His-tag in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, Cell culture supernatant, Cell Extract, and Tissue Extract. Quantitate His-tag with 36.9 ng/ml sensitivity.SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Competitive ELISA - His-tag ELISA Kit (ab322565)
Example of His-tag standard curve. OD (450 nm) values (mean +/- SD) are graphed.
Competitive ELISA - His-tag ELISA Kit (ab322565)
Interpolated concentration of native His-tag was measured in duplicate at different sample concentrations. Undiluted sample is E. coli cell extract expressing a protein with multiple tags at 7.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR + Enhancer.
Competitive ELISA - His-tag ELISA Kit (ab322565)
His-tag protein was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction PTR + Enhancer. Undiluted samples are 4.5% Human serum, plasma (citrate), plasma (EDTA), plasma (heparin), and urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - His-tag ELISA Kit (ab322565)
His-tag protein was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction PTR + Enhancer. Undiluted samples are 4.5% Mouse serum, plasma (citrate), plasma (EDTA), plasma (heparin), and urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - His-tag ELISA Kit (ab322565)
His-tag protein was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction PTR + Enhancer. Undiluted samples are 4.5% HEK293T cell supernatant, and 4.5% Jurkat cell supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - His-tag ELISA Kit (ab322565)
His-tag protein was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction PTR + Enhancer. Undiluted samples are 10 µg/mL HEK293T cell extract, 20 µg/mL CHO cell extract, 36 µg/mL Mouse brain extract, 110 µg/mL Mouse heart extract, and 90 µg/mL Mouse liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - His-tag ELISA Kit (ab322565)
His-tag protein was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction PTR + Enhancer. Undiluted samples are 4.5% LB Broth media, 2.25% yeast extraction buffer, 0.7% Lauryl Maltoside buffer, 115 mM urea Buffer, and 22.4 mM imidazole elution buffer. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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