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Horse IL-1 alpha ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-1 alpha in Horse in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

0.82 - 200 pg/mL

Sensitivity

= 0.82 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent B
1 x 15 mL
5X Assay Diluent D
1 x 15 mL
Anti-Horse IL-1 alpha coated Microplate (12 x 8 wells)
1 x 1 Unit
Biotinylated Anti-Horse IL-1 alpha Detection Antibody
2 x 1 Vial
Horse IL-1 alpha Standard (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB Substrate Solution
1 x 12 mL

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Horse IL-1 alpha ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-1 alpha in Horse in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

0.82 - 200 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.82 pg/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 141.2

Range

136 - 144 %

Sample type

Serum

Average %

= 143.5

Range

141 - 146 %

Sample type

Plasma

Average %

= 145.2

Range

141 - 148 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Horse IL-1alpha ELISA Kit is designed for the quantitative determination of Horse IL-1alpha in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for IL-1alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse IL-1alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Horse IL-1 alpha ELISA kit (ab272030), expandable thumbnail

    Sandwich ELISA - Horse IL-1 alpha ELISA kit (ab272030)

    Horse IL-1alpha Standard Curve.

    This standard curve is for demonstration only. A standard curve must be run with each assay.

    Horse IL-1alpha ELISA kit (ab272030) Standard curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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