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AB272030

Horse IL-1 alpha ELISA kit

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Horse IL-1 alpha ELISA kit is a Sandwich ELISA for the measurement of Horse IL-1 alpha in Horse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 alpha, IL-1 alpha, IL1A

1 Images
Sandwich ELISA - Horse IL-1 alpha ELISA kit (AB272030)
  • sELISA

Supplier Data

Sandwich ELISA - Horse IL-1 alpha ELISA kit (AB272030)

Horse IL-1alpha Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Horse IL-1alpha ELISA kit (ab272030) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Horse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.82 pg/mL

Range

0.82 - 200 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Horse IL-1alpha ELISA Kit is designed for the quantitative determination of Horse IL-1alpha in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for IL-1alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse IL-1alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "136 - 144 %", "average": "= 141.2" }, { "sample": "Serum", "range": "141 - 146 %", "average": "= 143.5" }, { "sample": "Plasma", "range": "141 - 148 %", "average": "= 145.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272030-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB272030-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB272030-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Horse IL-1 alpha coated Microplate (12 x 8 wells)", "number":"AB272030-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Horse IL-1 alpha Standard (Lyophilized)", "number":"AB272030-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB272030-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB272030-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB272030-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Horse IL-1 alpha Detection Antibody", "number":"AB272030-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB272030-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
See full target information IL1A
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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