Horse IL-1 alpha ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-1 alpha in Horse in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Horse
- Range
- 0.82 - 200 pg/mL
- Sensitivity
- = 0.82 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
Alternative names
Interleukin-1 alpha, IL-1 alpha, IL1A
What's included?
Recommended products
Horse IL-1 alpha ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-1 alpha in Horse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Horse
- Range
- 0.82 - 200 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.82 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 141.2 | Range 136 - 144 % |
Sample type Serum | Average % = 143.5 | Range 141 - 146 % |
Sample type Plasma | Average % = 145.2 | Range 141 - 148 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Horse IL-1alpha ELISA Kit is designed for the quantitative determination of Horse IL-1alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for IL-1alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse IL-1alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Terms & Conditions.
1 product image
Sandwich ELISA - Horse IL-1 alpha ELISA kit (ab272030)
Horse IL-1alpha Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse IL-1alpha ELISA kit (ab272030) Standard curve.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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