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Horse IL-2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-2 in Horse in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Horse
0.22 - 160 ng/mL
= 0.2 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
Horse IL-2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-2 in Horse in Plasma, Cell culture supernatant, Serum samples.
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Horse
0.22 - 160 ng/mL
Pre-coated microplate (12 x 8 well strips)
= 0.2 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 81.71 | Range 69 - 96 % |
Sample type Serum | Average % = 83.62 | Range 68 - 103 % |
Sample type Plasma | Average % = 79.81 | Range 69 - 92 % |
Blue Ice
-20°C
-20°C
-20°C
Horse IL-2 ELISA kit is designed for the quantitative determination of Equine IL-2 in cell culture supernatants, plasma and serum samples.
ΔNote: (Equine IL-2 concentration is low in normal serum/plasma and may not be detectable in this assay
This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Equine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects equine IL-2.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Equine IL-2 Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Equine IL-2 ELISA kit (ab272027) Standard curve.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com