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Horse IL-2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-2 in Horse in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

0.22 - 160 ng/mL

Sensitivity

= 0.2 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
500X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent B
1 x 15 mL
Anti-Horse IL-2 coated Microplate (12 x 8 wells)
1 x 1 Unit
Assay Diluent C
1 x 30 mL
Biotinylated Anti-Horse IL-2 Detection Antibody
2 x 1 Vial
Horse IL-2 Standard (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB Substrate Solution
1 x 12 mL

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Horse IL-2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse IL-2 in Horse in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

0.22 - 160 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.2 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 81.71

Range

69 - 96 %

Sample type

Serum

Average %

= 83.62

Range

68 - 103 %

Sample type

Plasma

Average %

= 79.81

Range

69 - 92 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Horse IL-2 ELISA kit is designed for the quantitative determination of Equine IL-2 in cell culture supernatants, plasma and serum samples.

ΔNote: (Equine IL-2 concentration is low in normal serum/plasma and may not be detectable in this assay

This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Equine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This kit detects equine IL-2.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Horse IL-2 ELISA kit (ab272027), expandable thumbnail

    Sandwich ELISA - Horse IL-2 ELISA kit (ab272027)

    Equine IL-2 Standard Curve.

    This standard curve is for demonstration only. A standard curve must be run with each assay.

    Equine IL-2 ELISA kit (ab272027) Standard curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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