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Horse SAA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse SAA in Horse in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

2.25 - 72 ng/mL

Assay time

1h 50m

Sensitivity

= 0.962 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Alternative names

What's included?

1 x 96 Tests
Components
100X Detection Antibody Conjugate
1 x 150 µL
100X HRP Streptavidin
1 x 150 µL
20X Wash Buffer Concentrate
1 x 50 mL
5X Diluent Concentrate
1 x 50 mL
Anti-Horse SAA ELISA Microplate
1 x 1 Unit
Chromogen Substrate Solution
1 x 12 mL
Horse SAA Calibrator (Lyophilized)
1 x 1 Vial
Stop Solution
1 x 12 mL

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Horse SAA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Horse SAA in Horse in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Horse

Range

2.25 - 72 ng/mL

Assay time

1h 50m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.962 ng/mL

General recovery

>= 85%

Precision

Intra assay

Sample

Serum

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Serum

n

0

mean

-

SD

-

C.V.

< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

>= 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C, Multi

Storage information

+4°C

Notes

SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in horse biological fluids.

In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SAA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Supplementary info

Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Activity summary

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

Associated diseases and disorders

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Horse SAA ELISA Kit (ab205087), expandable thumbnail

    Sandwich ELISA - Horse SAA ELISA Kit (ab205087)

    Representative standard curve using ab205087 Horse SAA ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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