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AB205087

Horse SAA ELISA Kit

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Horse SAA ELISA Kit is a Sandwich ELISA for the measurement of Horse SAA in Horse in Biofluids samples.

View Alternative Names

Serum amyloid A protein, SAA, SAA1

2 Images
Sandwich ELISA - Horse SAA ELISA Kit (AB205087)
  • sELISA

Supplier Data

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)

Representative standard curve using ab205087 Horse SAA ELISA Kit.

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)
  • sELISA

Supplier Data

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)

Representative standard curve using ab205087 Horse SAA ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Horse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.962 ng/mL

Range

2.25 - 72 ng/mL

Assay time

1h 50m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Primary Antibodies

AB199030

Anti-SAA1 + SAA2 antibody [EPR19235]

RabMAb|Recombinant

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in horse biological fluids.

In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SAA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205087-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB205087-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205087-CMP08", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Streptavidin", "number":"AB205087-CMP06", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X Detection Antibody Conjugate", "number":"AB205087-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205087-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205087-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Horse SAA ELISA Microplate", "number":"AB205087-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Horse SAA Calibrator (Lyophilized)", "number":"AB205087-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.
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Product protocols

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Target data

Major acute phase reactant. Apolipoprotein of the HDL complex.
See full target information SAA1
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websiteProtocolBooklet
en
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Product promise

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