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AB270882

HSP47 ELISA Kit

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HSP47 ELISA Kit is a single-wash 90-min Simplestep used to quantify HSP47 with a sensitivity of 119.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin

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Sandwich ELISA - HSP47 ELISA Kit (AB270882)
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Supplier Data

Sandwich ELISA - HSP47 ELISA Kit (AB270882)

Interpolated concentrations of native HSP47 in human HepG2, MCF7, and HeLa extract samples based on a 25 μg/mL extract load.

The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 14.52 ng/mL in HepG2, 14.19 ng/ml in MCF7, and 12.46 ng/mL in HeLa extract samples.

Sandwich ELISA - HSP47 ELISA Kit (AB270882)
  • sELISA

Supplier Data

Sandwich ELISA - HSP47 ELISA Kit (AB270882)

Example of HSP47 standard curve in Sample Diluent NS.

The HSP47 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - HSP47 ELISA Kit (AB270882)
  • sELISA

Supplier Data

Sandwich ELISA - HSP47 ELISA Kit (AB270882)

HSP47 standard curve comparison.

Standard Curve comparison between the original HSP47 SimpleStep ELISA kit (ab202407) and current HSP47 SimpleStep ELISA kit (ab270882).

Sandwich ELISA - HSP47 ELISA Kit (AB270882)
  • sELISA

Supplier Data

Sandwich ELISA - HSP47 ELISA Kit (AB270882)

Representative image of HSP47 ELISA Kit (ab270882)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X HSP47 Capture Antibody

- 10X HSP47 Detector Antibody

- HSP47 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - HSP47 ELISA Kit (AB270882)
  • sELISA

Supplier Data

Sandwich ELISA - HSP47 ELISA Kit (AB270882)

Interpolated concentrations of native HSP47 in human, mouse, and rat liver homogenate extract samples based on a 125 μg/mL extract load.

The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 2.16 ng/mL in human, 1.97 ng/ml in mouse, and 8.90 ng/mL in rat liver homogenate extract samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Citrate plasma, Serum, Cell Lysate

Reacts with

Mouse, Rat, Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 119.23 pg/mL

Range

218.75 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

HSP47 ELISA kit (ab270882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HSP47 protein in human serum and plasma (citrate) and human, mouse and rat cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate HSP47 with 119.23 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Heat Shock Protein47 (HSP47) is a collagen specific molecular chaperone protein found in the endoplasmic reticulum. HSP47 assists in the triple-helical formation of collagen and is part of the collagen quality control system. Collagen accounts for approximately 30% of the total protein found in vertebrates and is the major component responsible in providing structural support for a number of tissues. HSP47 can be found constitutively expressed in non-stressed cells associated with collagen expression. Increased HSP47 expression has been associated with increase collagen deposition in arteriosclerosis, myocardial infarction, hepatic fibrosis, renal fibrosis, and pulmonary fibrosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 90 %", "average": "= 89" }, { "sample": "Cell culture extracts", "range": "88 - 115 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "80 - 84 %", "average": "= 82" }, { "sample": "Cell Lysate", "range": "102 - 111 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270882-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270882-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270882-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270882-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270882-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB270882-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270882-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X HSP47 Detector Antibody", "number":"AB270882-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB270882-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB270882-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270882-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"HSP47 Lyophilized Recombinant Protein", "number":"AB270882-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X HSP47 Capture Antibody", "number":"AB270882-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.
Biological function summary

Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.

Pathways

Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.

Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.
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Product protocols

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Target data

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
See full target information SERPINH1
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websiteProtocolBooklet
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Product promise

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