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AB246543

Human 14-3-3 tau ELISA Kit

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Human 14-3-3 tau ELISA Kit is a single-wash 90-min Simplestep used to quantify Human 14-3-3 tau with a sensitivity of 6.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

2 Images
Sandwich ELISA - Human 14-3-3 tau ELISA Kit (AB246543)
  • sELISA

Supplier Data

Sandwich ELISA - Human 14-3-3 tau ELISA Kit (AB246543)

Interpolated concentrations of native 14-3-3 tau in human serum, plasma and cell culture supernatant samples.

The concentrations of 14-3-3 theta + tau were measured in duplicates, interpolated from the 14-3-3 theta + tau standard curves and corrected for sample dilution. Undiluted samples are as follows : A549 cell extract 0.6 μg/mL, Raji cell extract 0.3 μg/mL, and NIH/3T3 cell extract 0.6 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human 14-3-3 tau ELISA Kit (AB246543)
  • sELISA

Supplier Data

Sandwich ELISA - Human 14-3-3 tau ELISA Kit (AB246543)

Example of human 14-3-3 tau standard curve in 1X Cell Extraction Buffer PTR.

The 14-3-3 theta + tau standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.1 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human 14-3-3 theta + tau ELISA Kit (ab246543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of 37694 tau protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human 37694 tau with 6.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

14-3-3 Theta + Tau is a 245- amino acid containing adapter protein encoded by the gene YWHAQ. 14-3-3 tau belongs to the 14-3-3 family of proteins that are involved in a broad range of cell signaling pathways by binding to numerous other proteins, generally by recognition of either a phosphoserine or phosphothreonine motif. 14-3-3 theta + tau is highly conserved across species and has been shown to be involved in cellular processes including metabolism, protein trafficking, signal transduction, and apoptosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "80 - 107 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246543-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246543-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246543-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246543-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB246543-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human 14-3-3 tau Capture Antibody (Lyophilized)", "number":"AB246543-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human 14-3-3 tau Lyophilized Recombinant Protein", "number":"AB246543-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246543-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246543-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB246543-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246543-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human 14-3-3 tau Detector Antibody", "number":"AB246543-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 Theta also known as 14-3-3 protein theta is a member of the 14-3-3 protein family which consists of seven distinct isoforms in humans. It acts as an adaptor or scaffold aiding in the regulation of signal transduction pathways by binding to phosphoserine-containing proteins. The protein typically has a molecular mass of approximately 28-32 kDa. It is abundantly expressed in various tissues with a high concentration in the brain where it interacts with a number of neuronal proteins.
Biological function summary

14-3-3 Theta plays a significant role in cell cycle regulation apoptosis and brain development. It often forms part of larger protein complexes acting as a bridge to facilitate interactions between different proteins. The protein aids in maintaining cellular homeostasis and mediates various signal transduction processes by changing the conformation of its binding partners which influences their activity.

Pathways

14-3-3 Theta is actively involved in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation differentiation and survival. Through these pathways the protein interacts with a variety of partners including thr-308-phosphorylated Akt and Raf kinases. 14-3-3 Theta's ability to bind different phosphorylated targets enables it to influence diverse cellular processes within these pathways.

14-3-3 Theta has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's. Notably its interaction with tau protein plays an important role in Alzheimer's disease as abnormal phosphorylation and aggregation of tau is a characteristic of the disorder. Furthermore disruptions in 14-3-3 Theta function have connections to cancer by interacting with signaling proteins like the tumor suppressor p53 leading to altered cell growth and survival.
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Product protocols

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information 14-3-3 protein theta
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websiteProtocolBooklet
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Product promise

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