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AB314823

Human 14-3-3 theta ELISA Kit

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Human 14-3-3 theta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human 14-3-3 theta in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

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Sandwich ELISA - Human 14-3-3 theta ELISA Kit (AB314823)
  • sELISA

Supplier Data

Sandwich ELISA - Human 14-3-3 theta ELISA Kit (AB314823)

Example of standard curve using ab314823. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 24.5 pg/mL

Range

24.5 - 7000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human 14-3-3 theta ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human 14-3-3 theta protein in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human 14-3-3 theta protein coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "80 - 94 %", "average": "= 86.23" }, { "sample": "Plasma", "range": "94 - 104 %", "average": "= 99.86" }, { "sample": "Cell culture media", "range": "98 - 111 %", "average": "= 104.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314823-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"1400X HRP-Streptavidin", "number":"AB314823-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314823-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human 14-3-3 theta Protein Standard", "number":"AB314823-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314823-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314823-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human 14-3-3 theta Antibody-coated ELISA Plate", "number":"AB314823-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human 14-3-3 theta Detection Antibody", "number":"AB314823-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314823-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 theta also known as YWHAQ is a member of the 14-3-3 protein family with a mass of approximately 28 kDa. These proteins are known for binding phosphoserine-containing proteins. In the case of 14-3-3 theta it is involved in the regulation of a broad range of proteins by altering activity cellular localization and stability. This protein is widely expressed in many tissues but has high expression levels in the brain indicating its involvement in neural functions.
Biological function summary

14-3-3 theta plays key roles in various signaling pathways and cellular processes. It participates as a scaffold or adapter molecule in the formation of protein complexes aiding in signal transduction. Its ability to bind to numerous client proteins allows it to regulate biological processes such as apoptosis cell cycle progression and cellular stress responses. By facilitating these processes 14-3-3 theta maintains cellular homeostasis and proper cellular responses to environmental cues.

Pathways

14-3-3 theta integrally functions within the MAPK and PI3K/Akt signaling pathways. These pathways involve the regulation of cell growth survival and metabolism. In the MAPK pathway 14-3-3 theta collaborates with proteins like Raf and MEK assisting in the transduction of growth signals. In the PI3K/Akt pathway it interacts with Akt influencing cell survival and proliferation by modulating downstream targets.

Dysregulation of 14-3-3 theta has associations with neurodegenerative disorders like Alzheimer's disease and psychiatric disorders such as schizophrenia. In Alzheimer's disease interactions with tau protein are noteworthy which may affect tau phosphorylation and subsequent aggregation. In schizophrenia alterations in signaling pathways where 14-3-3 theta is an active participant might cause disruptions in neural signaling contributing to the disorder's pathophysiology.
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Product protocols

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information 14-3-3 protein theta
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