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AB309433

Human 15-PGDH ELISA Kit

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Human 15-PGDH ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human 15-PGDH in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PGDH1, SDR36C1, HPGD, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Eicosanoid/docosanoid dehydrogenase [NAD(+)], Prostaglandin dehydrogenase 1, Short chain dehydrogenase/reductase family 36C member 1

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Sandwich ELISA - Human 15-PGDH ELISA Kit (AB309433)
  • sELISA

Supplier Data

Sandwich ELISA - Human 15-PGDH ELISA Kit (AB309433)

Example of standard curve using ab309433. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Results type

Quantitative

Sensitivity

= 0.2 ng/mL

Range

0.2 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB187161

Anti-15-PGDH antibody [EPR14332-19]

RabMAb|Recombinant

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

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Assay Kits

AB155426

Human beta IG H3 ELISA Kit (TGFBI)

Sandwich ELISA - Human beta IG H3 ELISA Kit (TGFBI) (AB155426)

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Primary Antibodies

AB209584

PE Anti-Syndecan-1 antibody [EPR6454]

RabMAb|Recombinant

Flow Cytometry - PE Anti-Syndecan-1 antibody [EPR6454] (AB209584)

  • 4
  • 1
Assay Kits

AB273327

15-PGDH Activity Assay Kit (Fluorometric)

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human 15-PGDH ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human 15-PGDH in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human 15-PGDH coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "72 - 77 %", "average": "= 75.29" }, { "sample": "Plasma", "range": "67 - 77 %", "average": "= 71.08" }, { "sample": "Cell culture media", "range": "70 - 94 %", "average": "= 75.91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309433-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309433-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309433-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309433-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309433-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human 15-PGDH/HPGD Protein Standard", "number":"AB309433-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human 15-PGDH/HPGD Detection Antibody", "number":"AB309433-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human 15-PGDH/HPGD Antibody-coated ELISA Plate", "number":"AB309433-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309433-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309433-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-PGDH also known as 15-hydroxyprostaglandin dehydrogenase PGDH or 15-PGDH protein is an enzyme with a molecular mass of approximately 29 kDa. This enzyme catalyzes the oxidation of the 15-hydroxyl group in prostaglandins specifically converting Prostaglandin E2 (PGE2) into its inactive form. Expression occurs in various tissues including the lung placenta and colonic epithelium. Its activity levels can vary depending on the tissue context and physiological conditions marking its importance in local regulation.
Biological function summary

15-PGDH regulates prostaglandin levels which are lipid compounds playing roles in inflammation and homeostasis. This enzyme does not associate with protein complexes but serves a standalone function in metabolic pathways. It maintains a balance between prostaglandin synthesis and degradation ensuring that inflammatory responses do not exceed what is necessary. The enzyme's activity directly reduces available prostaglandins which in turn dampens related signaling pathways.

Pathways

Prostaglandins and this enzyme are part of arachidonic acid metabolism and the Cyclooxygenase (COX) pathway. These pathways play key roles in inflammation and tissue homeostasis. 15-PGDH interacts with other enzymes like COX-2 as it acts downstream to mitigate PGE2 levels generated by COX enzymes. This diminishes the signaling of other elements such as the AMPA receptors involved in neuronal plasticity and pain perception showcasing the enzyme's regulatory scope within biological systems.

15-PGDH plays a role in colon cancer and pulmonary inflammation. Low expression or activity of this enzyme is associated with increased PGE2 levels contributing to cancer progression and chronic inflammation. This dysregulation may involve proteins like COX-2 where the imbalance between synthesis and degradation leads to elevated prostaglandin signaling. Understanding 15-PGDH functions offers insights into therapeutic potentials making inhibitors and potentiators of interest for treatment of these conditions.
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Product protocols

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Target data

Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed : 10837478, PubMed : 16757471, PubMed : 16828555, PubMed : 21916491, PubMed : 25586183, PubMed : 8086429). Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed : 15574495, PubMed : 25586183). Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation (PubMed : 16757471, PubMed : 22844113).
See full target information HPGD
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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