Human ABCA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ABCA1 with a sensitivity of 0.06 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.16 - 10 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.06 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
Alternative names
ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1
What's included?
Recommended products
Human ABCA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ABCA1 with a sensitivity of 0.06 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.16 - 10 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.06 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 7.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 91 - 107 % |
Sample type EDTA Plasma | Average % = 102 | Range 98 - 107 % |
Sample type Cell culture media | Average % = 93 | Range 92 - 95 % |
Sample type Heparin Plasma | Average % = 99 | Range 92 - 109 % |
Sample type Citrate plasma | Average % = 99 | Range 89 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ABCA1 ELISA kit (ab284614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ABCA1 protein in human serum, plasma, media, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ABCA1 with 0.06 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
- Biological function summary
ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.
- Pathways
Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.
- Associated diseases and disorders
ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
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6 product images
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Example of human ABCA1 standard curve in Sample Diluent NS.
Example of human ABCA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Example of human ABCA1 standard curve in 1X Cell Extraction Buffer PTR.
Example of human ABCA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Interpolated concentrations of human ABCA1 in THP-1 cell extract.
Interpolated concentration of native ABCA1 was measured in duplicate at different sample concentrations. Undiluted samples are 100 μg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Interpolated concentrations of human ABCA1 in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).
Interpolated concentration of native ABCA1 was measured in duplicate at different sample concentrations. Undiluted samples are serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Interpolated concentrations of ABCA1 in normal human serum donors.
Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean ABCA1 concentration was determined to be 0.96 ng/mL with a range of 0.85 – 1.14 ng/mL.
Sandwich ELISA - Human ABCA1 ELISA Kit (ab284614)
Interpolated concentrations of ABCA1 in human THP-1 cell extracts treated with TO901317 and untreated.
The concentration of ABCA1 was measured in duplicates in human THP-1 cell extracts treated with TO901317 (1 μM, 24hr) and untreated based on a 100 μg/mL extract load. The mean concentration was determined to be 11.0 ng/mL in treated THP-1 cell extract and 1.10 ng/mL in untreated THP-1 cell extract.
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