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AB270207

Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase)

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Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) is a single-wash 90-min Simplestep used to quantify Human ABO (Histo-Blood Group ABO System Transferase) with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase, GTA, B transferase, GTB, ABO

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Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)
  • sELISA

Supplier Data

Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)

Interpolated concentrations of native ABO in human serum and plasma samples.

The concentrations of ABO were measured in duplicates, interpolated from the ABO standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ABO concentration was determined to be 768 pg/mL in serum, 976 pg/mL in plasma (citrate), 824 pg/mL in plasma (EDTA), and 1,059 pg/mL in plasma (heparin).

Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)
  • sELISA

Supplier Data

Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)

Example of human ABO standard curve in 1X Cell Extraction Buffer PTR.

The ABO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (ab270207) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ABO (Histo-Blood Group ABO System Transferase) protein in edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ABO (Histo-Blood Group ABO System Transferase) with 8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ABO, Histo-Blood Group ABO System Transferase, is a glucosyltransferase that is the key determinant of an individual’s blood type. This enzyme transfers UDP-GalNAC or UDP-Gal to H-antigen which results in A, B, or AB blood type. The antibodies for this kit were raised against recombinant protein representing the lumenal domain of the mature protein. The immunogen for this kit does not share high sequence similarity with mouse or rat ABO protein and is not expected to cross-react. However, this protein is homologous to primate ABO.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 96" }, { "sample": "EDTA Plasma", "range": null, "average": "= 101" }, { "sample": "Heparin Plasma", "range": null, "average": "= 102" }, { "sample": "Citrate plasma", "range": null, "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ABO blood group system frequently referred to as ABO is a critical component in determining blood type. It involves the presence of antigens on the surface of red blood cells which are glycosyltransferase enzymes that transfer sugar residues onto the H antigen. The gene responsible for this system is located on chromosome 9 and the variations in the gene produce the A B and O blood types. These glycoproteins and glycolipids confer blood group specificity. ABO is found in the erythrocytic lineage and is an integral part of the blood plasma context.
Biological function summary

The ABO proteins modulate immune response mechanisms. They participate in the formation of specific antigens and antibodies that define the ABO blood group system. These proteins are not part of a larger complex but interact closely with components in the immune system. In individuals the presence or absence of antigen types influences immune activity and the antigen-antibody structure is essential in transfusion medicine.

Pathways

Researchers link the ABO system with several immune response pathways. The system integrates into the complement system and humoral immunity affecting compatibility in blood transfusions. It is indirectly related to proteins in the complement cascade and these interactions facilitate or hinder immune reactions during transfusions and organ transplants. The pathway involvement highlights the system’s significance in cellular communication and immune mobilization.

ABO incompatibility can lead to hemolytic disease of the newborn and transfusion reactions. In such contexts the presence of incompatible ABO antigens triggers immune responses resulting in adverse events. It also indirectly connects to diseases like gastric cancer where blood type prevalence indicates varying disease risk profiles. The interaction with proteins such as the Rh factor can exacerbate these conditions showing how ABO status affects susceptibility and symptom severity.
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Product protocols

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Target data

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens : A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of N-acetyl-alpha-D-galactosamine (GalNAc)) or to the B antigen (by addition of galactose (Gal)), whereas O individuals lack such activity and express the H antigen precursor unmodified.. Catalyzes the transfer of GalNAc or Gal in an alpha1,3 linkage to the terminal Gal of all five types of naturally occurring H antigens, forming the antigenic structures of the A and B blood groups.
See full target information Histo-blood group ABO system transferase
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websiteProtocolBooklet
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Product promise

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