AB303748

Human ABP1 ELISA Kit

Recombinant
SimpleStep
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Human ABP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ABP1 with a sensitivity of 83.489 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ABP, ABP1, DAO, DAO1, AOC1, Diamine oxidase [copper-containing], Diamine oxidase, Amiloride-binding protein, Amiloride-binding protein 1, Amine oxidase copper domain-containing protein 1, Histaminase, Kidney amine oxidase, KAO, KDAO

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Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

sELISA

Supplier Data

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Interpolated concentrations of human ABP1 in THP-1 cell culture supernatant.

Interpolated concentration of native ABP1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% THP-1 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Example of human ABP1 standard curve in Sample Diluent NS.

Example of human ABP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 83.489 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ABP1 SimpleStep ELISA® kit (ab303748) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ABP1 protein in serum, plasma (EDTA) and cell culture supernatant. Quantitate Human ABP1 with 83.489 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.24" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.11" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 106 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "80 - 99 %", "average": "= 88" }, { "sample": "Serum", "range": "86 - 96 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303748-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303748-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303748-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303748-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB303748-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303748-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ABP1 Lyophilized Recombinant Protein", "number":"AB303748-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ABP1 Capture Antibody", "number":"AB303748-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303748-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303748-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ABP1 Detector Antibody", "number":"AB303748-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABP1 also known as Auxin Binding Protein 1 is a protein with a molecular mass of approximately 22 kDa. This protein primarily functions as an auxin receptor in plants mediating various cellular responses to the plant hormone auxin. ABP1 locates mainly in the endoplasmic reticulum and is present across various plant tissues. The protein has a significant affinity for auxin and triggers specific intracellular signaling cascades essential for growth and development processes.

Biological function summary

ABP1 plays an important role in controlling cell elongation and division and it works in tandem with other auxin transporters. The protein functions within a broader receptor complex interacting with other proteins to modify plant cell physiology. Its action facilitates cell wall modification which directly influences the plant's response to environmental and endogenous signals. Through these interactions ABP1 significantly contributes to regulating the plant's phototropic and gravitropic responses.

Pathways

The operation of ABP1 integrates into the auxin signaling pathway and is associated with the regulation of gene expression. It links to TIR1 an important F-box protein in the SCF complex involved in the auxin signal transduction pathway. Together ABP1 and TIR1 coordinate to modulate the stability of transcription factors affecting downstream gene expression necessary for plant growth and development.

ABP1 itself mostly pertains to plant biology but its function offers insights into plant growth abnormalities. Disruptions in ABP1 function may result in disorders like auxin-insensitive phenotypes leading to defective root and shoot development. Additionally ABP1's interaction with PIN proteins responsible for auxin efflux creates potential for research into correcting growth defects in crops making it a critical focus in agricultural science.

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Product protocols

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Target data

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 12072962, PubMed : 19764817, PubMed : 239684, PubMed : 8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed : 12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed : 12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed : 12072962, PubMed : 239684).

See full target information AOC1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ABP1 ELISA Kit

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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