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AB315049

Human ACAT1 ELISA Kit

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Human ACAT1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ACAT1 with a sensitivity of 35.966 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ACAT, MAT, ACAT1, Acetoacetyl-CoA thiolase, T2

2 Images
Sandwich ELISA - Human ACAT1 ELISA Kit (AB315049)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACAT1 ELISA Kit (AB315049)

Example of human ACAT1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ACAT1 ELISA Kit (AB315049)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACAT1 ELISA Kit (AB315049)

Interpolated concentration of native ACAT1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 30 µg/mL, HepG2 cell extract 15 µg/mL, heart tissue extract 15 µg/mL, and liver tissue extract 2 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 35.966 ng/mL

Range

0.063 - 4 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human ACAT1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACAT1 protein in Cell Lysate, Cell Lysate, Tissue extracts, Tissue extracts. Quantitate Human ACAT1 with 35.966 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "98 - 129 %", "average": "= 113" }, { "sample": "Tissue Extracts", "range": "108 - 132 %", "average": "= 116" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315049-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315049-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315049-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315049-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315049-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ACAT1 Lyophilized Recombinant Protein", "number":"AB315049-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB315049-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACAT1 Capture Antibody", "number":"AB315049-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315049-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315049-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACAT1 Detector Antibody", "number":"AB315049-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315049-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAT1 also known as acyl-CoA:cholesterol acyltransferase 1 is an enzyme that catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty acyl-CoA. It has a molecular mass of approximately 50 kDa. ACAT1 is present in the endoplasmic reticulum membrane and is expressed widely including in liver adrenal glands and macrophages. The enzyme's activity is significant in lipid metabolism storing cholesterol as cholesteryl esters.
Biological function summary

ACAT1 plays a central role in cellular cholesterol homeostasis and in preventing cholesterol-induced cytotoxicity by converting free cholesterol into inert cholesteryl esters for storage. It functions as a homotetramer suggesting that it works as a part of a larger protein complex. ACAT1 influences lipid accumulation within cells and mediates the regulation of cholesterol levels which is vital for maintaining membrane integrity and cellular function.

Pathways

ACAT1 participates in the cholesterol metabolism pathway and the lipid biosynthesis pathway. It interacts with proteins such as LDLR (low-density lipoprotein receptor) by controlling the availability of free cholesterol within cells. These pathways are critical in maintaining lipid balance and influencing lipoprotein metabolism. ACAT1's enzymatic activity impacts how cells manage cholesterol influx and storage linking to broader lipid regulation networks.

ACAT1 is associated with atherosclerosis and Alzheimer's disease. The conversion of cholesterol into cholesteryl esters affects the development of atherosclerotic plaques contributing to cardiovascular disease risk. In Alzheimer's disease ACAT1 may influence the accumulation of amyloid-beta a protein linked to the pathology of the disease. The protein APP (amyloid precursor protein) connects to ACAT1 as disturbances in cholesterol metabolism can affect amyloid-beta production linking this enzyme to neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed : 1715688, PubMed : 7728148, PubMed : 9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed : 1715688, PubMed : 7728148, PubMed : 9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed : 17371050). Thereby, it plays a major role in ketone body metabolism (PubMed : 1715688, PubMed : 17371050, PubMed : 7728148, PubMed : 9744475).
See full target information ACAT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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