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AB235649

Human ACE2 ELISA Kit

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(1 Review)

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(27 Publications)

Human ACE2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ACE2 with a sensitivity of 1052 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

View Alternative Names

UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase

5 Images
ELISA - Human ACE2 ELISA Kit (AB235649)
  • ELISA

Supplier Data

ELISA - Human ACE2 ELISA Kit (AB235649)
Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)

Interpolated concentrations of native ACE2 in human urine samples.

The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 75.24 ng/mL in urine.

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)

Representative image of Human ACE2 ELISA Kit (ab235649)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human ACE2 ELISA Kit Capture Antibody

- 10X Human ACE2 Detector Antibody

- 10X Human ACE2 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human ACE2 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)

Example of human ACE2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit (AB235649)

Interpolated concentrations of native ACE2 in human lung, heart, fetal intestine, kidney, and testes samples.

The concentrations of ACE2 were measured in duplicate and interpolated from the ACE2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 26 ng/mL in lung extract, 31 ng/mL in heart extract, 115 ng/mL in fetal intestine, 94 ng/mL in kidney, and 146 ng/mL in testes extract.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1052 pg/mL

Range

3.98 - 255 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ACE2 ELISA Kit ab235649 is a rapid single-wash 90-min Sandwich ELISA to measure Human ACE2 in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 1052 pg/mL.

How the assay works

Human ACE2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ACE2 ELISA Kit ab235649 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ACE2 antibody [EPR20239-267] - BSA and Azide free (Capture) ab244777
- Anti-ACE2 antibody [EPR20239-236] - BSA and Azide free (Detector) ab245050

ACE2 is a key enzyme in the renin-angiotensin system which regulates blood pressure. Specifically, ACE2 cleaves the terminal nonapeptide from angiotensin I or the terminal septapeptide from angiotensin II to create angiotensin[1-9] and angiotensin[1-7], respectively. The produced peptides work to oppose the effects of angiotensin II and act as a vasodilator. This enzyme is currently under investigation as a potential target for reducing instances of heart failure.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 99" }, { "sample": "EDTA Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Urine", "range": "85 - 89 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "110 - 116 %", "average": "= 112" }, { "sample": "Tissue Extracts", "range": "100 - 108 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "98 - 100 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
Biological function summary

The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.

Pathways

ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.

ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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Product protocols

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Target data

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed : 27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed : 10924499, PubMed : 10969042, PubMed : 11815627, PubMed : 14504186, PubMed : 19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed : 10969042, PubMed : 11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed : 11815627, PubMed : 27217402, PubMed : 28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed : 18424768, PubMed : 19185582).. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.. Isoform 2. Non-functional as a carboxypeptidase.. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
See full target information ACE2
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40227199

2025

Impact of Glucose, Inflammation and Phytochemicals on , and Glucose Transporter Gene Expression in Human Intestinal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rizliya Visvanathan,Michael J Houghton,Gary Williamson

Narra J 4:e746 PubMed39280273

2024

Perindopril decreases angiotensin-converting enzyme 2 (ACE2) expression in human adipocytes exposed to SARS-CoV-2 S1 spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Primasitha M Harsoyo,Meity Ardiana,Hanestya O Hermawan,Yeni Purnamasari,Faizal A Anandita

BioFactors (Oxford, England) 50:1268-1286 PubMed38886986

2024

The flavonoid quercetin decreases ACE2 and TMPRSS2 expression but not SARS-CoV-2 infection in cultured human lung cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael James Houghton,Eglantine Balland,Matthew James Gartner,Belinda Jane Thomas,Kanta Subbarao,Gary Williamson

Cerebrovascular diseases (Basel, Switzerland) 54:105-111 PubMed38471482

2024

Plasma Soluble Angiotensin-Converting Enzyme 2 and Risk of Recurrent Stroke: A Nested Case-Control Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xue,Mingming Shi,Qin Xu,Anxin Wang,Xue Jiang,Jinxi Lin,Xia Meng,Hao Li,Lemin Zheng,Yongjun Wang,Jie Xu

Narra J 3:e284 PubMed38455635

2024

Effect of SARS-CoV-2 spike protein exposure on ACE2 and interleukin 6 productions in human adipocytes: An in-vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Meity Ardiana,I Gr Suryawan,Hanestya O Hermawan,Primasitha M Harsoyo,Aisya A Shafira,Faizal A Anandita

BioMed research international 2023:5705076 PubMed37929242

2023

Intronic Variants of the Angiotensin-Converting Enzyme 2 Gene Modulate Plasma ACE2 Levels and Possibly Confer Protection against Severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Rubaiat Ahmed,Abdullah Al Saba,Anik Paul,Jasmin Nur,Md Sohrab Alam,Sajib Chakraborty,Md Zakir Hossain Howlader,Laila N Islam,A H M Nurun Nabi

iScience 26:107470 PubMed37609639

2023

Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Onodera,Jady Liang,Yuchong Li,Bryan Griffin,Thenuka Thanabalasingam,Cong Lu,JiaYi Zhu,Mingyao Liu,Theo Moraes,Wenhua Zheng,Jasmin Khateeb,Julie Khang,Yongbo Huang,Mirjana Jerkic,Masaki Nakane,Andrew Baker,Beverley Orser,Ya-Wen Chen,Gerald Wirnsberger,Josef M Penninger,Ori D Rotstein,Arthur S Slutsky,Yimin Li,Samira Mubareka,Haibo Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2207249 PubMed37096860

2023

Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Liu,Lunzhi Yuan,Jijing Chen,Yali Zhang,Peiwen Chen,Ming Zhou,Jiaxuan Xie,Jian Ma,Jianzhong Zhang,Kun Wu,Qiyi Tang,Quan Yuan,Huachen Zhu,Tong Cheng,Yi Guan,Gang Liu,Ningshao Xia

Pharmacy practice 21:2761 PubMed37090459

2023

The effect of omega-3 supplements on the serum levels of ACE/ACE2 ratio as a potential key in cardiovascular disease: A randomized clinical trial in participants with vitamin D deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Sara M Daboul,Mohammad Abusamak,Beisan A Mohammad,Ahmad R Alsayed,Maha Habash,Ibrahim Mosleh,Sami Al-Shakhshir,Reem Issa,Mahmoud Abu-Samak

Frontiers in immunology 14:1105237 PubMed36936972

2023

Diversity of immune responses in children highly exposed to SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

María Úbeda,María Del Carmen Maza,Pilar Delgado,Lydia Horndler,David Abia,Laura García-Bermejo,Sergio Serrano-Villar,Cristina Calvo,Ugo Bastolla,Talia Sainz,Manuel Fresno
View all publications
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