Name: Human ACE2 ELISA Kit
SKU: ab235649
Rating: 5 (1 reviews)

Human ACE2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ACE2 in Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Alternative names=Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase, UNQ868/PRO1885, ACE2

Detection method: Colorimetric
Sample types: Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 3.98 - 255 ng/mL
Assay time: 1h 30m
Sensitivity: = 1052 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

rec-h ACE2

What's included?

1 x 96 Tests

Components

10X Human ACE2 Capture Antibody

1 x 600 µL

10X Human ACE2 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

50X Cell Extraction Enhancer Solution (ab193971)

1 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 10 mL

Antibody Diluent 4BI

1 x 6 mL

Human ACE2 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 12 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 3.98 - 255 ng/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 1052 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Extract	n 8	mean -	SD -	C.V. 2.3

Inter assay

Sample	n	mean	SD	C.V.
Sample Extract	n 3	mean -	SD -	C.V. 3.2

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 99	Range
Sample type EDTA Plasma	Average % = 91	Range 90 - 92 %
Sample type Urine	Average % = 88	Range 85 - 89 %
Sample type Cell culture media	Average % = 112	Range 110 - 116 %
Sample type Tissue Extracts	Average % = 104	Range 100 - 108 %
Sample type Heparin Plasma	Average % = 82	Range 81 - 84 %
Sample type Citrate plasma	Average % = 99	Range 98 - 100 %

Storage

Shipped at conditions: Blue Ice
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human ACE2 ELISA Kit (ab235649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of native ACE2 protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACE2 with 1052 pg/ml sensitivity.

Note: Plasma, serum, and cell culture media samples were validated with spiked recombinant ACE2. Neat serum from ten individual healthy human female/male donors was measured in duplicate and all values were below the detectable range of the assay.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ACE2 is a key enzyme in the renin-angiotensin system which regulates blood pressure. Specifically, ACE2 cleaves the terminal nonapeptide from angiotensin I or the terminal septapeptide from angiotensin II to create angiotensin[1-9] and angiotensin[1-7], respectively. The produced peptides work to oppose the effects of angiotensin II and act as a vasodilator. This enzyme is currently under investigation as a potential target for reducing instances of heart failure.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human ACE2 ELISA Kit

Reactivity data

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