Human ACE2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ACE2 in Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- Narra J 3:e2842024Effect of SARS-CoV-2 spike protein exposure on ACE2 and interleukin 6 productions in human adipocytes: An in-vitro study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeity Ardiana,I Gr Suryawan,Hanestya O Hermawan,Primasitha M Harsoyo,Aisya A Shafira,Faizal A AnanditaPubMed 38455635
- BioMed research international 2023:57050762023Intronic Variants of the Angiotensin-Converting Enzyme 2 Gene Modulate Plasma ACE2 Levels and Possibly Confer Protection against Severe COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRubaiat Ahmed,Abdullah Al Saba,Anik Paul,Jasmin Nur,Md Sohrab Alam,Sajib Chakraborty,Md Zakir Hossain Howlader,Laila N Islam,A H M Nurun NabiPubMed 37929242
- iScience 26:1074702023Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Onodera,Jady Liang,Yuchong Li,Bryan Griffin,Thenuka Thanabalasingam,Cong Lu,JiaYi Zhu,Mingyao Liu,Theo Moraes,Wenhua Zheng,Jasmin Khateeb,Julie Khang,Yongbo Huang,Mirjana Jerkic,Masaki Nakane,Andrew Baker,Beverley Orser,Ya-Wen Chen,Gerald Wirnsberger,Josef M Penninger,Ori D Rotstein,Arthur S Slutsky,Yimin Li,Samira Mubareka,Haibo ZhangPubMed 37609639
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22072492023Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Liu,Lunzhi Yuan,Jijing Chen,Yali Zhang,Peiwen Chen,Ming Zhou,Jiaxuan Xie,Jian Ma,Jianzhong Zhang,Kun Wu,Qiyi Tang,Quan Yuan,Huachen Zhu,Tong Cheng,Yi Guan,Gang Liu,Ningshao XiaPubMed 37096860
- Pharmacy practice 21:27612023The effect of omega-3 supplements on the serum levels of ACE/ACE2 ratio as a potential key in cardiovascular disease: A randomized clinical trial in participants with vitamin D deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara M Daboul,Mohammad Abusamak,Beisan A Mohammad,Ahmad R Alsayed,Maha Habash,Ibrahim Mosleh,Sami Al-Shakhshir,Reem Issa,Mahmoud Abu-SamakPubMed 37090459
- Frontiers in immunology 14:11052372023Diversity of immune responses in children highly exposed to SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Úbeda,María Del Carmen Maza,Pilar Delgado,Lydia Horndler,David Abia,Laura García-Bermejo,Sergio Serrano-Villar,Cristina Calvo,Ugo Bastolla,Talia Sainz,Manuel FresnoPubMed 36936972
- Viruses 14:2022Expression of SARS-Cov-2 Entry Factors in Patients with Chronic Hepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Rosso,Cristina Demelas,Greta Agostini,Maria Lorena Abate,Marta Vernero,Gian Paolo Caviglia,Daphne D'Amato,Angelo Armandi,Marta Tapparo,Marta Guariglia,Giulia Troshina,Alessandro Massano,Antonella Olivero,Aurora Nicolosi,Antonella Zannetti,Rinaldo Pellicano,Alessia Ciancio,Giorgio Maria Saracco,Davide Giuseppe Ribaldone,Elisabetta Bugianesi,Sharmila FagooneePubMed 36366497
- Frontiers in cellular and infection microbiology 12:9429512022GDF15 and ACE2 stratify COVID-19 patients according to severity while ACE2 mutations increase infection susceptibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargalida Torrens-Mas,Catalina M Perelló-Reus,Neus Trias-Ferrer,Lesly Ibargüen-González,Catalina Crespí,Aina Maria Galmes-Panades,Cayetano Navas-Enamorado,Andres Sanchez-Polo,Javier Piérola-Lopetegui,Luis Masmiquel,Lorenzo Socias Crespi,Carles Barcelo,Marta Gonzalez-FreirePubMed 35937703
- Frontiers in immunology 13:8365162022ACE2 Serum Levels as Predictor of Infectability and Outcome in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Del Carmen Maza,María Úbeda,Pilar Delgado,Lydia Horndler,Miguel A Llamas,Hisse M van Santen,Balbino Alarcón,David Abia,Laura García-Bermejo,Sergio Serrano-Villar,Ugo Bastolla,Manuel FresnoPubMed 35401548
- Frontiers in bioscience (Landmark edition) 27:72022David Hunter Hubel, the , and SARS-CoV-2 infection of the human visual system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWalter J LukiwPubMed 35090312
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.98 - 255 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1052 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2. Non-functional as a carboxypeptidase. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Alternative names
UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
What's included?
Recommended products
Human ACE2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ACE2 in Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.98 - 255 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1052 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 2.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99 | Range |
Sample type EDTA Plasma | Average % = 91 | Range 90 - 92 % |
Sample type Urine | Average % = 88 | Range 85 - 89 % |
Sample type Cell culture media | Average % = 112 | Range 110 - 116 % |
Sample type Tissue Extracts | Average % = 104 | Range 100 - 108 % |
Sample type Heparin Plasma | Average % = 82 | Range 81 - 84 % |
Sample type Citrate plasma | Average % = 99 | Range 98 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ACE2 ELISA Kit (ab235649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of native ACE2 protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACE2 with 1052 pg/ml sensitivity.
Note: Plasma, serum, and cell culture media samples were validated with spiked recombinant ACE2. Neat serum from ten individual healthy human female/male donors was measured in duplicate and all values were below the detectable range of the assay.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ACE2 is a key enzyme in the renin-angiotensin system which regulates blood pressure. Specifically, ACE2 cleaves the terminal nonapeptide from angiotensin I or the terminal septapeptide from angiotensin II to create angiotensin[1-9] and angiotensin[1-7], respectively. The produced peptides work to oppose the effects of angiotensin II and act as a vasodilator. This enzyme is currently under investigation as a potential target for reducing instances of heart failure.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
- Biological function summary
The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.
- Pathways
ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.
- Associated diseases and disorders
ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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3 product images
Sandwich ELISA - Human ACE2 ELISA Kit (ab235649)
Example of human ACE2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ACE2 ELISA Kit (ab235649)
Interpolated concentrations of native ACE2 in human urine samples.
The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 75.24 ng/mL in urine.
Sandwich ELISA - Human ACE2 ELISA Kit (ab235649)
Interpolated concentrations of native ACE2 in human lung, heart, fetal intestine, kidney, and testes samples.
The concentrations of ACE2 were measured in duplicate and interpolated from the ACE2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 26 ng/mL in lung extract, 31 ng/mL in heart extract, 115 ng/mL in fetal intestine, 94 ng/mL in kidney, and 146 ng/mL in testes extract.
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