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AB327289

Human ACE2 ELISA Kit, Chemiluminescent

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Human ACE2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human ACE2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.93 - 2040 ng/ml with a sensitivity of 0.24 ng/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)

Interpolated concentrations of native ACE2 in human lung heart fetal intestine kidney and testes samples.

The concentrations of ACE2 were measured in duplicate and interpolated from the ACE2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean ACE2 concentration was determined to be 26 ng/mL in lung extract 31 ng/mL in heart extract 115 ng/mL in fetal intestine 94 ng/mL in kidney and 146 ng/mL in testes extract.

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)

Interpolated concentrations of native ACE2 in human urine samples.

The concentrations of ACE2 were measured in duplicates interpolated from the ACE2 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean ACE2 concentration was determined to be 75.24 ng/mL in urine.

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)
  • sELISA

Lab

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)

Dynamic range comparison between the Chemiluminescent Human ACE2 SimpleStep ELISA and colorimetric Human ACE2 SimpleStep ELISA (ab235649). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)
  • sELISA

Lab

Sandwich ELISA - Human ACE2 ELISA Kit, Chemiluminescent (AB327289)

Standard curve comparison between the Chemiluminescent Human ACE2 SimpleStep ELISA and colorimetric Human ACE2 SimpleStep ELISA (ab235649) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Urine, Cell culture media, Tissue Extracts, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.24 ng/mL

Range

0.93 - 2040 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ACE2 ELISA Kit, Chemiluminescent (ab327289) is a rapid single-wash sandwich 55-min ELISA to measure Human ACE2 in Serum, EDTA Plasma, Urine, Cell culture media, Tissue Extracts, Heparin Plasma, Citrate plasma with a sensitivity of 0.24 ng/mL.

How the assay works

Human ACE2 ELISA Kit, Chemiluminescent (ab327289) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ACE2 (ab327289) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ACE2 antibody [EPR20239-267] - BSA and Azide free (Capture) ab244777
- Anti-ACE2 antibody [EPR20239-236] - BSA and Azide free (Detector) ab245050

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 99" }, { "sample": "EDTA Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Urine", "range": "85 - 89 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "110 - 116 %", "average": "= 112" }, { "sample": "Tissue Extracts", "range": "100 - 108 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "98 - 100 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed : 27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed : 10924499, PubMed : 10969042, PubMed : 11815627, PubMed : 14504186, PubMed : 19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed : 10969042, PubMed : 11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed : 11815627, PubMed : 27217402, PubMed : 28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed : 18424768, PubMed : 19185582).. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.. Isoform 2. Non-functional as a carboxypeptidase.. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
See full target information ACE2

Alternative Names

UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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