AB318934

Human Acetylcholinesterase ELISA Kit - Extracellular

Recombinant
SimpleStep
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Human Acetylcholinesterase ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Acetylcholinesterase with a sensitivity of 20.314 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

Acetylcholinesterase, AChE, ACHE

5 Images

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

sELISA

Supplier Data

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Interpolated concentrations of human Acetylcholinesterase in plasma (EDTA) in 96-well vs. 384-well plates. Interpolated concentration of native Acetylcholinesterase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% or plasma (EDTA). The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Interpolated concentrations of human Acetylcholinesterase in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and CSF. Interpolated concentration of native Acetylcholinesterase was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), plasma (heparin), and CSF. The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Interpolated concentrations of Acetylcholinesterase in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Acetylcholinesterase concentration was determined to be 3,863 pg/mL with a range of 2,902 – 5,543 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Example of human Acetylcholinesterase standard curve in Sample Diluent NS. Example of human Acetylcholinesterase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Example of human Acetylcholinesterase standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human Acetylcholinesterase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20.314 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Acetylcholinesterase SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Acetylcholinesterase protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human Acetylcholinesterase with 20.314 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "71 - 118 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "97 - 113 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "76 - 92 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "101 - 120 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "90 - 116 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "89 - 120 %", "average": "= 106" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318934-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB318934-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318934-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318934-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318934-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB318934-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318934-CMP05" }, { "size":"10 x 2 Vial", "name":"Human Acetylcholinesterase Lyophilized Recombinant Protein", "number":"AB318934-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Acetylcholinesterase Capture Antibody", "number":"AB318934-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human Acetylcholinesterase Detector Antibody", "number":"AB318934-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318934-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab318934-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318934-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB318934-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318934-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB318934-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318934-CMP05" }, { "size":"2 x 1 Vial", "name":"Human Acetylcholinesterase Lyophilized Recombinant Protein", "number":"AB318934-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Acetylcholinesterase Capture Antibody", "number":"AB318934-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB318934-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human Acetylcholinesterase Detector Antibody", "number":"AB318934-CMP02" }, { "size":"1 x 384 Wells", "name":"384 well CaptSure™ microplates", "number":"AB318934-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318934-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318934-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318934-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318934-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318934-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318934-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Acetylcholinesterase Lyophilized Recombinant Protein", "number":"AB318934-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Acetylcholinesterase Capture Antibody", "number":"AB318934-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB318934-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Acetylcholinesterase Detector Antibody", "number":"AB318934-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318934-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.

Biological function summary

Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.

Pathways

Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.

Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.

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Product protocols

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Target data

Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.

See full target information ACHE

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Acetylcholinesterase ELISA Kit - Extracellular

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Sandwich ELISA - Human Acetylcholinesterase ELISA Kit - Extracellular (AB318934)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise