AB324922

Human ACSL4/FACL4 ELISA Kit

SimpleStep
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Human ACSL4/FACL4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ACSL4/FACL4 with a sensitivity of 86.5pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders.

-Knock out validated for guaranteed specificity

View Alternative Names

ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4

3 Images

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Example of human ACSL4/FACL4 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Interpolated concentration of native ACSL4/FACL4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell extract 2.5 µg/mL and liver tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Interpolated concentration of native ACSL4/FACL4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 62.5 µg/mL wild type and ACSL4/FACL4 knockout A-431 cell extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. The mean concentration was determined to be 11,031 pg/mL in wild type extract and undetectable in knockout extract. The ACSL4/FACL4 A-431 knockout cell line is available as ab269613.

Key facts

Detection method

Colorimetric

Sample types

Cell Extract, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 86.5 pg/mL

Range

218.75 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ACSL4/FACL4 ELISA Kit (ab324922) is a rapid single-wash sandwich 90-min ELISA to measure Human ACSL4/FACL4 in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 86.5 pg/ml.

How the assay works

Human ACSL4/FACL4 ELISA Kit (ab324922) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ACSL4/FACL4 (ab324922) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "103 - 117 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "88 - 99 %", "average": "= 95" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324922-10x96Tests", "assetComponentDetails": [ { "size":"20 x 1 Vial", "name":"Human ACSL4/FACL4 Lyophilized Recombinant Protein", "number":"AB324922-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human ACSL4/FACL4 Detector Antibody", "number":"AB324922-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human ACSL4/FACL4 Capture Antibody", "number":"AB324922-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324922-CMP11" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324922-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324922-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324922-CMP07" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB324922-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB324922-CMP13" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324922-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324922-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324922-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human ACSL4/FACL4 Lyophilized Recombinant Protein", "number":"AB324922-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACSL4/FACL4 Capture Antibody", "number":"AB324922-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACSL4/FACL4 Detector Antibody", "number":"AB324922-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324922-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB324922-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324922-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324922-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324922-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324922-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324922-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324922-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324922-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed : 21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed : 21242590).

See full target information ACSL4

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websiteProtocolBooklet

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Product promise

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