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AB267814

Human ACTH ELISA Kit (adrenocorticotropic hormone)

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Human ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min Simplestep used to quantify Human ACTH (adrenocorticotropic hormone) with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH

4 Images
ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)
  • ELISA

Supplier Data

ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)
Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)

Interpolated concentrations of spike ACTH in human serum and plasma.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%.

Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)

Interpolated concentrations of native ACTH in human plasma (EDTA) (from late-term pregnant donors) samples.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 30 pg/mL in plasma (EDTA) (from late-term pregnant donors).

Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)
  • sELISA

Supplier Data

Sandwich ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)

Example of human ACTH standard curve in 1X Cell Extraction Buffer PTR.

The ACTH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.2 pg/mL

Range

2.5 - 160 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ACTH ELISA Kit (adrenocorticotropic hormone) ab267814 is a rapid single-wash 90-min Sandwich ELISA to measure Human ACTH (adrenocorticotropic hormone)in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.2 pg/mL.

How the assay works

Human ACTH (adrenocorticotropic hormone)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ACTH ELISA Kit (adrenocorticotropic hormone) ab267814 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "82 - 85 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "87 - 89 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267814-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267814-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267814-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267814-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267814-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267814-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ACTH (adrenocorticotropic hormone) Lyophilized Protein", "number":"AB267814-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB267814-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACTH (adrenocorticotropic hormone) Capture Antibody", "number":"AB267814-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ACTH (adrenocorticotropic hormone) Detector Antibody", "number":"AB267814-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB267814-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267814-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenocorticotropic hormone (ACTH) also known as corticotropin is a peptide hormone secreted by the pituitary gland. It is a 39-amino acid polypeptide with a molecular mass of approximately 4540 Da. ACTH is primarily expressed in the anterior pituitary gland and smaller amounts can be found in the central nervous system. Its production and secretion are tightly regulated by corticotropin-releasing hormone (CRH) through a feedback mechanism involving the hypothalamic-pituitary-adrenal (HPA) axis.
Biological function summary

Adrenocorticotropic hormone stimulates the adrenal cortex to produce and release glucocorticoids especially cortisol. ACTH is not part of a larger complex but acts as a singular hormone in its pathway. The hormone also influences the expression of certain enzymes important for steroid biosynthesis. In addition to its role in the adrenal cortex ACTH can mildly interact with melanocortin receptors on adipose tissues contributing to lipolysis and inflammatory processes.

Pathways

Adrenocorticotropic hormone functions in the HPA axis one of the body's key stress response systems. The hormone also involves in the regulation of the corticosteroid biosynthesis pathway. The primary protein it interacts with in these processes is cortisol which in turn regulates many physiological processes including immune response and metabolism. ACTH and cortisol form a feedback loop where elevated cortisol levels inhibit the release of both CRH and ACTH.

Increased or decreased levels of adrenocorticotropic hormone can result in disorders such as Cushing's disease and Addison's disease. Cushing's disease is characterized by overproduction of ACTH often due to pituitary adenomas leading to excess cortisol production. Conversely in Addison’s disease inadequate ACTH or adrenal cortex dysfunction leads to insufficient cortisol production. Both conditions highlight the disrupted feedback relationship between ACTH and cortisol resulting in significant clinical symptoms that require careful management.
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Product protocols

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Target data

See full target information ACTH
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websiteProtocolBooklet
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