AB119568
Human Activin A ELISA Kit is a sandwich ELISA designed to quantify Human Activin A with a sensitivity of 12 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 62.5 - 4000 pg/mL
View Alternative Names
Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Activin A ELISA Kit (AB119568)
Representative Standard Curve using ab119568.
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Reactivity data
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Product details
Human Activin A ELISA Kit ab119568 is a sandwich ELISA to measure Human Activin A in serum, cell culture supernatant, tissue extracts, other biological fluids with a sensitivity of 12 pg/ml.
How the assay works
A Activin A specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection monoclonal antibody from mouse, specific for Activin A is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Activin A amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Activin A ELISA Kit ab119568 protocol summary
1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
How the assay works
A Activin A specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection monoclonal antibody from mouse, specific for Activin A is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Activin A amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Activin A ELISA Kit ab119568 protocol summary
1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Activin A also known by its gene name INHBA is a protein involved in various mechanical processes in the human body. This protein forms part of the transforming growth factor-beta (TGF-β) superfamily and has a molecular weight of approximately 25 kDa for its dimeric forms. Activin A is expressed in many tissues including the pituitary gland reproductive organs and placenta. Researchers often use techniques like the Activin A ELISA test kit to measure its concentration in biological samples.
Biological function summary
Activin A plays a significant role in regulating cell differentiation proliferation and apoptosis. As part of a complex Activin A interacts with specific receptors on cell surfaces initiating intracellular signaling cascades that influence these vital processes. It acts as a growth factor in the reproductive system influencing follicle-stimulating hormone (FSH) secretion and is important in embryonic development and wound healing.
Pathways
Activin A participates in pathways such as the TGF-β signaling pathway and the SMAD protein signaling pathway. Within these pathways it interacts with receptor proteins like type I and type II Activin receptors and downstream SMAD proteins. These interactions enable Activin A to regulate gene expression relevant to various cellular functions.
Altered Activin A levels have associations with conditions like cancer and fibrotic diseases. Its overexpression links to some cancer types including colorectal and prostate cancer where it influences tumor growth and metastasis. Moreover its interaction with proteins like myostatin may contribute to muscle wasting disorders. The dysregulation of these pathways highlights the significance of Activin A in pathophysiology.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.. Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis (PubMed : 3194407, PubMed : 16440334). Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression (PubMed : 10652306, PubMed : 24018044). Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation (PubMed : 16440334). Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway (PubMed : 34948289). In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling (PubMed : 26333933).. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 24: PubMed36768622
2023
TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva.
Applications
Unspecified application
Species
Unspecified reactive species
Ruben D de Ruiter,Lisanne E Wisse,Ton Schoenmaker,Maqsood Yaqub,Gonzalo Sánchez-Duffhues,E Marelise W Eekhoff,Dimitra Micha
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