Human Activin AB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Activin AB in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.5 - 2000 pg/mL
- Sensitivity
- = 8.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Alternative names
Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA
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Human Activin AB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Activin AB in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.5 - 2000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8.5 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101.8 | Range 88 - 125 % |
Sample type Plasma | Average % = 100.5 | Range 80 - 110 % |
Sample type Cell culture media | Average % = 120.3 | Range 98 - 145 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Activin AB ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Activin AB in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Activin AB coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP- 1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Inhibin beta A also known as activin beta A chain and inhibin A is a protein with a significant role in the regulation of diverse physiological processes. This protein has a molecular mass of approximately 45 kDa and originates primarily from gonadal tissues but also from the placenta pituitary gland and other tissues. Inhibin beta A consists of several subunits that can dimerize to form both activins and inhibins depending on the pairing with other subunits like the alpha chain.
- Biological function summary
Activin beta A chain plays an important role in the modulation of follicle-stimulating hormone (FSH) regulation cellular growth and differentiation. It often functions as part of a complex with activin and inhibin proteins influencing reproductive hormone levels and participating in tissue repair and inflammation processes. Inhibin beta A forms activin A when paired with another inhibin beta A subunit or inhibin A when paired with an inhibin alpha subunit therefore contributing to the modulation of FSH-releasing protein activity.
- Pathways
Activin and inhibin proteins participate in the regulation of the TGF-beta signaling pathway which is essential for reproductive and developmental processes. In this context inhibin beta A interacts functionally with proteins such as follistatin and FSH-releasing protein which modulate pathways that influence follicle maturation and hormone secretion. The protein's role in these pathways highlights its importance in orchestrating hormonal responses and facilitating proper cellular communication within the endocrine system.
- Associated diseases and disorders
Inhibin beta A becomes relevant in the context of reproductive health and oncology. Changes in its expression can associate with disorders like polycystic ovary syndrome (PCOS) and certain cancers particularly ovarian cancer where it may alter hormonal regulation and tumor progression. Related proteins such as inhibin B and follistatin also play roles in these conditions further complicating the pathways and the potential development of therapeutic interventions that target inhibin beta A and its network of interactions.
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1 product image
Sandwich ELISA - Human Activin AB ELISA Kit (ab314824)
Example of standard curve using ab314824. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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