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AB314824

Human Activin AB ELISA Kit

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Human Activin AB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Activin AB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA

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Sandwich ELISA - Human Activin AB ELISA Kit (AB314824)
  • sELISA

Supplier Data

Sandwich ELISA - Human Activin AB ELISA Kit (AB314824)

Example of standard curve using ab314824. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.5 pg/mL

Range

8.5 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Activin AB ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Activin AB in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Activin AB coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP- 1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

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Recovery

[ { "sample": "Serum", "range": "88 - 125 %", "average": "= 101.8" }, { "sample": "Plasma", "range": "80 - 110 %", "average": "= 100.5" }, { "sample": "Cell culture media", "range": "98 - 145 %", "average": "= 120.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314824-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314824-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314824-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Activin AB Detection Antibody", "number":"AB314824-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Activin AB Protein Standard", "number":"AB314824-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314824-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314824-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314824-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314824-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Activin AB Antibody-coated ELISA Plate", "number":"AB314824-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin beta A also known as activin beta A chain and inhibin A is a protein with a significant role in the regulation of diverse physiological processes. This protein has a molecular mass of approximately 45 kDa and originates primarily from gonadal tissues but also from the placenta pituitary gland and other tissues. Inhibin beta A consists of several subunits that can dimerize to form both activins and inhibins depending on the pairing with other subunits like the alpha chain.
Biological function summary

Activin beta A chain plays an important role in the modulation of follicle-stimulating hormone (FSH) regulation cellular growth and differentiation. It often functions as part of a complex with activin and inhibin proteins influencing reproductive hormone levels and participating in tissue repair and inflammation processes. Inhibin beta A forms activin A when paired with another inhibin beta A subunit or inhibin A when paired with an inhibin alpha subunit therefore contributing to the modulation of FSH-releasing protein activity.

Pathways

Activin and inhibin proteins participate in the regulation of the TGF-beta signaling pathway which is essential for reproductive and developmental processes. In this context inhibin beta A interacts functionally with proteins such as follistatin and FSH-releasing protein which modulate pathways that influence follicle maturation and hormone secretion. The protein's role in these pathways highlights its importance in orchestrating hormonal responses and facilitating proper cellular communication within the endocrine system.

Inhibin beta A becomes relevant in the context of reproductive health and oncology. Changes in its expression can associate with disorders like polycystic ovary syndrome (PCOS) and certain cancers particularly ovarian cancer where it may alter hormonal regulation and tumor progression. Related proteins such as inhibin B and follistatin also play roles in these conditions further complicating the pathways and the potential development of therapeutic interventions that target inhibin beta A and its network of interactions.
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Product protocols

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Target data

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
See full target information Inhibin beta A chain
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websiteProtocolBooklet
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Product promise

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