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AB277463

Human ACY-1 ELISA

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Human ACY-1 ELISA is a Sandwich (quantitative) ELISA for the measurement of Human ACY-1 ELISA in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Aminoacylase-1, ACY-1, N-acyl-L-amino-acid amidohydrolase, ACY1

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ELISA - Human ACY-1 ELISA (AB277463)
  • ELISA

Supplier Data

ELISA - Human ACY-1 ELISA (AB277463)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.19 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human ACY-1 ELISA Kit (ab277463) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ACY-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human ACY-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ACY-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ACY-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ACY-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 120 %", "average": "= 86.56" }, { "sample": "Cell culture media", "range": "72 - 121 %", "average": "= 90.4" }, { "sample": "Plasma", "range": "71 - 108 %", "average": "= 87.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277463-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277463-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277463-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human ACY-1 coated Microplate", "number":"AB277463-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human ACY-1 Antibody", "number":"AB277463-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ACY-1 standard protein (Lyophilized)", "number":"AB277463-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277463-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB277463-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277463-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277463-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ACY-1 also known as aminoacylase 1 or ACY plays a significant role in the deacetylation process of N-acyl amino acids. ACY-1 is a zinc-binding enzyme and has a molecular weight of approximately 45 kDa. This enzyme is expressed in various tissues including the liver and kidney. ACY-1 is essential for amino acid metabolism by catalyzing the hydrolysis of N-acyl-L-amino acids into L-amino acids and free fatty acids.
Biological function summary

The aminoacylase 1 enzyme functions in detoxification and metabolic regulation. It is not typically part of large protein complexes but acts independently to regulate amino acid and acetyl-CoA pathways. The activity facilitates the recycling of acyl groups and amino acids which is important for maintaining cellular homeostasis.

Pathways

Aminoacylase 1 is involved in amino acid catabolism and lipid metabolism pathways. In these processes ACY-1 interacts with proteins such as acetyl-CoA synthetase in the conversion of excess acyl-CoAs into free fatty acids and CoA preventing the build-up of toxic metabolites. It also has an indirect relationship with the urea cycle by ensuring the availability of free amino acids for further metabolism.

Alterations in ACY-1 function relate to neurological disorders and metabolic syndromes. For instance defects in ACY-1 have been associated with Canavan disease a condition affecting the brain. The protein's activity imbalance might also link to metabolic disorders where its interaction with proteins like aspartoacylase comes into play further affecting the acetylation status and metabolic regulation.
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Product protocols

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Target data

Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.
See full target information Aminoacylase-1
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websiteProtocolBooklet
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