Human ADAM22 ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ADAM22 - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed:19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
Alternative names
MDC2, ADAM22, Disintegrin and metalloproteinase domain-containing protein 22, ADAM 22, Metalloproteinase-disintegrin ADAM22-3
What's included?
Human ADAM22 ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ADAM22 - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18.2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 3.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 4.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 97 - 109 % |
Sample type Citrate plasma | Average % = 94 | Range 91 - 96 % |
Sample type EDTA Plasma | Average % = 100 | Range 94 - 104 % |
Sample type Heparin Plasma | Average % = 88 | Range 83 - 92 % |
Sample type Cell culture supernatant | Average % = 99 | Range 93 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ADAM22 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ADAM22 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human ADAM22 with 18.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
- Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Interpolated concentration of native ADAM22 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Example of human ADAM22 standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ADAM22 concentration was determined to be 11.35 ng/mL with a range of 6.46 – 18.13 ng/mL.
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Interpolated concentration of native ADAM22 was measured in duplicate at different sample concentrations. Undiluted samples are 10% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Interpolated concentration of native ADAM22 was measured in duplicate at different sample concentrations. Undiluted samples are 10% SH-SY5Y cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (ab324133)
Example of human ADAM22 standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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