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AB193690

Human ADAM9 ELISA Kit

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Human ADAM9 ELISA Kit is a Sandwich ELISA for the measurement of Human ADAM9 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

KIAA0021, MCMP, MDC9, MLTNG, ADAM9, Disintegrin and metalloproteinase domain-containing protein 9, ADAM 9, Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase

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Sandwich ELISA - Human ADAM9 ELISA Kit (AB193690)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAM9 ELISA Kit (AB193690)

Standard Curve in Assay Diluent B using ab193690 ADAM9 Human ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Human ADAM9 ELISA Kit (AB193690)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAM9 ELISA Kit (AB193690)

Standard Curve in Assay Diluent A using ab193690 ADAM9 Human ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 45 pg/mL

Range

45 - 12000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's ADAM9 Human ELISA Kit (ab193690) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ADAM9 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human ADAM9 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ADAM9 present in the samples. The wells are washed and biotinylated anti-Human ADAM9 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ADAM9 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 104 %", "average": "= 96.06" }, { "sample": "Plasma", "range": "81 - 101 %", "average": "= 91.5" }, { "sample": "Cell culture media", "range": "114 - 130 %", "average": "= 122.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193690-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193690-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193690-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human ADAM9 Microplate (12 strips x 8 wells)", "number":"AB193690-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193690-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ADAM9 Standards (lyophilized)", "number":"AB193690-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193690-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody ADAM9 (biotinylated anti-Human ADAM9)", "number":"AB193690-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193690-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB193690-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM9 also known as Meltrin gamma is a member of the ADAM (a disintegrin and metalloproteinase) family characterized by both proteolytic and adhesive properties. The ADAM9 protein weighs approximately 84 kDa and is expressed in various tissues including the brain throat and lungs. It plays an important role in cellular processes such as cell-cell and cell-matrix interactions due to its disintegrin domain.
Biological function summary

ADAM9 participates in numerous essential cellular functions particularly in cell surface protein shedding and proteolytic activities. It is part of the protease complex involved in shedding epidermal growth factor receptor (EGFR) ligands. This action impacts cell signaling pathways that contribute to processes such as cell proliferation migration and invasion.

Pathways

Many studies link ADAM9 to pathways involving cell signal modulation and extracellular matrix remodeling. It participates in the EGFR signaling pathway significantly affecting cell behavior. ADAM9 interacts with other proteins such as integrins which facilitate changes in cell dynamics and communication.

Evidence implicates ADAM9 in several cancers including breast cancer and prostate cancer. It plays a role in cancer development by promoting cell invasion and metastasis. ADAM9 is also associated with Alzheimer's disease through interactions with proteins like APP (amyloid precursor protein) suggesting its involvement in neuronal degradation and plaque formation.
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Product protocols

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Target data

Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins.. Isoform 2. May act as alpha-secretase for amyloid precursor protein (APP).
See full target information ADAM9
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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