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AB213751

Human ADAMTS1 ELISA Kit

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(1 Publication)

Human ADAMTS1 ELISA Kit is a Sandwich ELISA for the measurement of Human ADAMTS1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

KIAA1346, METH1, ADAMTS1, A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1

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Sandwich ELISA - Human ADAMTS1 ELISA Kit (AB213751)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS1 ELISA Kit (AB213751)

Human ADAMTS1 ELISA Kit (ab213751) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

93.8 - 6000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human ADAMTS1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213751) is designed for the quantitative measurement of Human ADAMTS1 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS1 amount of sample captured in plate.

ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. The ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2886 pg/mL", "standardDeviation": "207.79", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "2843 pg/mL", "standardDeviation": "213.22", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "132 pg/mL", "standardDeviation": "10.56", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "732 pg/mL", "standardDeviation": "51.97", "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "145 pg/mL", "standardDeviation": "8.84", "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "681 pg/mL", "standardDeviation": "37.45", "coefficientOfVariability": "= 5.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS1 short for a disintegrin and metalloproteinase with thrombospondin motifs 1 is an enzyme with a mass of around 110 kDa. Researchers often study this enzyme for its role in protein degradation and modification. ADAMTS1 is found in various tissues including the heart lungs and kidneys. This protein has several aliases sometimes referred to as ADAMTS-1 or ADAM-TS1. Its primary function involves processing extracellular matrix components such as aggrecan and versican which contribute to tissue remodeling.
Biological function summary

The influence of ADAMTS1 extends to various physiological and developmental processes. This enzyme participates in the modulation of angiogenesis inflammation and reproductive functions. It operates within a complex of metalloproteinases coordinating the breakdown of matrix proteins. Through these activities ADAMTS1 maintains tissue integrity and homeostasis. It particularly plays an essential role in the regulation of proteoglycans which are critical in cellular communication and structure.

Pathways

The actions of ADAMTS1 intersect within the extracellular matrix organization and integrin signaling pathways. These pathways are important for cell adhesion and migration contributing to tissue morphogenesis and repair. ADAMTS1 shows interaction with proteins like integrins and other matrix metalloproteinases establishing a network critical for cellular structural changes and signal transduction. These interactions ensure balanced tissue development and response to environmental changes.

The dysfunction of ADAMTS1 has links to conditions like osteoarthritis and cancer. In osteoarthritis inadequate regulation of matrix components by ADAMTS1 can lead to joint degradation. In cancer its dysregulation may influence tumor growth and metastasis. ADAMTS1 interacts with other proteins implicated in these diseases such as aggrecanases in osteoarthritis and integrins in cancer. These connections highlight the potential role of ADAMTS1 as a therapeutic target in managing these complex diseases.
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Product protocols

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Target data

Metalloprotease which cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover (By similarity). Also cleaves COMP (PubMed : 39672391). Has angiogenic inhibitor activity (PubMed : 10438512). May play a critical role in follicular rupture (By similarity).
See full target information ADAMTS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39859253

2025

Predictive Value of Selected Plasma Biomarkers in the Assessment of the Occurrence and Severity of Coronary Artery Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Małgorzata Wojciechowska,Michał Nizio,Katarzyna Wróbel,Karol Momot,Katarzyna Czarzasta,Krzysztof Flis,Maciej Zarębiński
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Product promise

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