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AB234559

Human ADAMTS13 ELISA Kit

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(9 Publications)

Human ADAMTS13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ADAMTS13 with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

C9orf8, UNQ6102/PRO20085, ADAMTS13, A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease

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ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • ELISA

Supplier Data

ELISA - Human ADAMTS13 ELISA Kit (AB234559)
Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Interpolated concentrations of native ADAMTS13 in human liver tissue extract based on a 1,000 μg/mL extract load.

The concentrations of ADAMTS13 were measured in duplicate and interpolated from the ADAMTS13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 0.364 ng/mL in human liver tissue extract.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Linearity of dilution in Sample Diluent NS.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native ADAMTS13 was measured in serum and plasma samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Recombinant ADAMTS13 was spiked into neat RPMI cell culture media containing 10% FBS and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Linearity of dilution in 1X Cell Extraction Buffer PTR.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native ADAMTS13 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in 1X Cell Extraction Buffer PTR.

The ADAMTS13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Human ASAMTS13 Competitor Std Curve Comparison.

Standard curve comparison between human ADAMTS13 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Interpolated concentrations of native ADAMTS13 in human serum and plasma samples.

The concentrations of ADAMTS13 were measured in duplicates, interpolated from the ADAMTS13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 200, plasma (heparin) 1 : 200 and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 1,082 ng/mL in neat serum, 963.3 ng/mL in neat plasma (citrate), 760.3 ng/mL in neat plasma (heparin), and 681.5 ng/mL in neat plasma (EDTA).

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in 1X Cell Extraction Buffer PTR.

The ADAMTS13 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 990.8 ng/mL with a range of 687.3 – 1,346 ng/mL.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in Sample Diluent NS.

The ADAMTS13 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Supplier Data

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in Sample Diluent NS.

The ADAMTS13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Interpolated concentrations of human ADAMTS13 in human liver extract in 96-well vs. 384-well plates.

Interpolated concentration of native ADAMTS13 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of human ADAMTS13 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Example of human ADAMTS13 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of human ADAMTS13 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit (AB234559)

Interpolated concentrations of human ADAMTS13 in serum in 96-well vs. 384-well plates.

Interpolated concentration of native ADAMTS13 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 200 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16 pg/mL

Range

0.125 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ADAMTS13 ELISA Kit ab234559 is a rapid single-wash 90-min Sandwich ELISA to measure Human ADAMTS13 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 16 pg/mL.

How the assay works

Human ADAMTS13 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ADAMTS13 ELISA Kit ab234559 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human ADAMTS13 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ADAMTS13 antibody [EPR21716-142] - BSA and Azide free (Capture) ab244747
- Anti-ADAMTS13 antibody [EPR21716-158] - BSA and Azide free (Detector) ab245017

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 115 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "98 - 104 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "103 - 111 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "100 - 105 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "97 - 100 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS13 also known as von Willebrand factor-cleaving protease (VWFCP) is a zinc-containing metalloprotease with a molecular mass of approximately 190 kDa. This protein belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family and exhibits a complex structure with specific domains including a metalloprotease and a disintegrin-like domain. ADAMTS13 is produced mainly in the liver and circulates in the blood plasma. Research shows that it primarily facilitates the cleavage of von Willebrand factor (vWF) a large multimeric protein essential for blood clotting.
Biological function summary

ADAMTS13 plays a role in regulating the size and function of von Willebrand factor (vWF) ensuring proper hemostatic balance. The enzyme prevents the accumulation of ultra-large vWF multimers which can lead to spontaneous platelet aggregation and thrombus formation. Although ADAMTS13 acts independently its function is closely linked to the dynamics of vWF in response to vascular injury. Properly functioning ADAMTS13 aids in maintaining normal blood flow by preventing unnecessary clot formation in the bloodstream.

Pathways

ADAMTS13 is critical in the coagulation and hemostatic pathways. It operates by modulating the activity of vWF which plays an essential role in platelet adhesion and aggregation forming part of the coagulation cascade. A significant relationship exists between ADAMTS13 and vWF in these pathways as the protease controls vWF multimer size directly impacting clot formation. In the context of hemostatic balance ADAMTS13 intersects with factors like thrombin and fibrinogen which further contribute to clotting processes.

ADAMTS13 deficiency or dysfunction is associated with thrombotic thrombocytopenic purpura (TTP) a rare but severe blood disorder. In TTP the reduced activity of ADAMTS13 leads to an over-accumulation of ultra-large vWF multimers resulting in excessive platelet aggregation and microvascular thrombosis. Another related condition is atypical hemolytic uremic syndrome (aHUS) where abnormal ADAMTS13 activity might exacerbate the disease pathology. Both disorders highlight the critical need for balanced ADAMTS13 function in maintaining vascular health and highlight its potential as a therapeutic target.
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Product protocols

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Target data

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
See full target information ADAMTS13
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38892211

2024

Inflammatory and Cardiovascular Biomarkers to Monitor Fabry Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián Alonso-Núñez,Tania Pérez-Márquez,Marta Alves-Villar,Carlos Fernández-Pereira,Julián Fernández-Martín,Alberto Rivera-Gallego,Cristina Melcón-Crespo,Beatriz San Millán-Tejado,Aurora Ruz-Zafra,Remedios Garofano-López,Rosario Sánchez-Martínez,Elena García-Payá,Manuel López-Mendoza,Ignacio Martín-Suárez,Saida Ortolano

Biomedicines 10: PubMed35740360

2022

Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hultström,Karin Fromell,Anders Larsson,Barbro Persson,Bo Nilsson,Susan E Quaggin,Christer Betsholtz,Robert Frithiof,Miklos Lipcsey,Marie Jeansson

Anesthesiology 137:67-78 PubMed35412597

2022

Suppression of Fibrinolysis and Hypercoagulability, Severity of Hypoxemia, and Mortality in COVID-19 Patients: A Retrospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin M Corey,Lyra B Olson,Ibtehaj A Naqvi,Sarah A Morrison,Connor Davis,Shahid M Nimjee,Loretta G Que,Robin E Bachelder,Bryan D Kraft,Lingye Chen,Smita K Nair,Jerrold H Levy,Bruce A Sullenger

JACC. Basic to translational science 7:247-261 PubMed35411318

2022

Hyperadhesive von Willebrand Factor Promotes Extracellular Vesicle-Induced Angiogenesis: Implication for LVAD-Induced Bleeding.

Applications

Unspecified application

Species

Unspecified reactive species

Mengchen Yang,Katie L Houck,Xinlong Dong,Maria Hernandez,Yi Wang,Sriram S Nathan,Xiaoping Wu,Vahid Afshar-Kharghan,Xiaoyun Fu,Miguel A Cruz,Jianning Zhang,Angelo Nascimbene,Jing-Fei Dong

Frontiers in medicine 8:817305 PubMed35087853

2022

The Impact of Age and BMI on the VWF/ADAMTS13 Axis and Simultaneous Thrombin and Plasmin Generation in Hospitalized COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kiruphagaran Thangaraju,Upendra Katneni,Imo J Akpan,Kenichi Tanaka,Tiffany Thomas,Saini Setua,Julie A Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David J Roh,Chiara Moriconi,James C Zimring,Angelo D'Alessandro,Steven L Spitalnik,Richard O Francis,Paul W Buehler

Cells 10: PubMed34571942

2021

Biological and Clinical Factors Contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Tiffany Thomas,Imo J Akpan,Julie A Reisz,Francesca I Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David Roh,Chiara Moriconi,James C Zimring,Eldad A Hod,Steven L Spitalnik,Paul W Buehler,Richard O Francis

Diagnostics (Basel, Switzerland) 11: PubMed34206615

2021

A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo Gaude,Barbara Nogueira,Marcos Ladreda Mochales,Sheila Graham,Sarah Smith,Lisa Shaw,Sara Graziadio,Gonzalo Ladreda Mochales,Philip Sloan,Joshua D Bernstock,Shashank Shekhar,Toby I Gropen,Christopher I Price

Research square : PubMed34013258

2021

Biological and Clinical Factors contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Imo Akpan,Tiffany Thomas,Julie Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Wei,Jacob Valk,Krystalyn Hudson,David Roh,Chiara Moriconi,James Zimring,Eldad Hod,Steven Spitalnik,Paul Buehler,Richard Francis

Critical care explorations 2:e0194 PubMed32904031

2020

Endothelial Injury and Glycocalyx Degradation in Critically Ill Coronavirus Disease 2019 Patients: Implications for Microvascular Platelet Aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas D Fraser,Eric K Patterson,Marat Slessarev,Sean E Gill,Claudio Martin,Mark Daley,Michael R Miller,Maitray A Patel,Claudia C Dos Santos,Karen J Bosma,David B O'Gorman,Gediminas Cepinskas
View all publications
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