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AB325628

Human ADAMTS13 ELISA Kit, Chemiluminescent

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Human ADAMTS13 ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human ADAMTS13 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 27.43 - 60000 pg/ml with a sensitivity of 7.07 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

C9orf8, UNQ6102/PRO20085, ADAMTS13, A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease

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Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

Standard curve comparison between the Chemiluminescent Human ADAMTS13 SimpleStep ELISA and colorimetric Human ADAMTS13 SimpleStep ELISA (ab234559) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

Standard curve comparison between the Chemiluminescent Human ADAMTS13 SimpleStep ELISA and colorimetric Human ADAMTS13 SimpleStep ELISA (ab234559) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

Dynamic range comparison between the Chemiluminescent Human ADAMTS13 SimpleStep ELISA, colorimetric Human ADAMTS13 SimpleStep ELISA (ab234559), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

The concentrations of ADAMTS13 were measured in duplicates, interpolated from the ADAMTS13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 200, plasma (heparin) 1 : 200 and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 1,082 ng/mL in neat serum, 963.3 ng/mL in neat plasma (citrate), 760.3 ng/mL in neat plasma (heparin), and 681.5 ng/mL in neat plasma (EDTA).

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

Interpolated concentrations of native ADAMTS13 in human liver tissue extract based on a 1,000 μg/mL extract load. The concentrations of ADAMTS13 were measured in duplicate and interpolated from the ADAMTS13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 0.364 ng/mL in human liver tissue extract.

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)
  • sELISA

Lab

Sandwich ELISA - Human ADAMTS13 ELISA Kit, Chemiluminescent (AB325628)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 990.8 ng/mL with a range of 687 – 1,346 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.07 pg/mL

Range

27.43 - 60000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ADAMTS13 ELISA Kit, Chemiluminescent (ab325628) is a rapid single-wash sandwich 55 min ELISA to measure Human ADAMTS13 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Tissue Extract with a sensitivity of 7.07 pg/mL.

How the assay works

Human ADAMTS13 ELISA Kit, Chemiluminescent (ab325628) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ADAMTS13 (ab325628) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ADAMTS13 antibody [EPR21716-142] - BSA and Azide free (Capture) ab244747
- Anti-ADAMTS13 antibody [EPR21716-158] - BSA and Azide free (Detector) ab245017
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 115 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "100 - 105 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "103 - 111 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "98 - 104 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "93 - 100 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
See full target information ADAMTS13
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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