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AB313504

Human ADAMTS15 ELISA Kit

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Human ADAMTS15 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ADAMTS15 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, ADAMTS15

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Sandwich ELISA - Human ADAMTS15 ELISA Kit (AB313504)
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Supplier Data

Sandwich ELISA - Human ADAMTS15 ELISA Kit (AB313504)

Example of standard curve using ab313504. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.45 ng/mL

Range

0.45 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ADAMTS15 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ADAMTS15 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ADAMTS15 coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "76 - 94 %", "average": "= 85.27" }, { "sample": "Plasma", "range": "87 - 124 %", "average": "= 101.1" }, { "sample": "Serum", "range": "88 - 124 %", "average": "= 102.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313504-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313504-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ADAMTS15 Protein Standard", "number":"AB313504-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ADAMTS15 Detection Antibody", "number":"AB313504-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ADAMTS15 Antibody-coated ELISA Plate", "number":"AB313504-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313504-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313504-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313504-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313504-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313504-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS15 also known as A Disintegrin And Metalloproteinase with Thrombospondin motifs 15 is a member of the ADAMTS family of zinc-dependent metalloproteases. It is involved in the degradation of extracellular matrix components especially proteoglycans. This protease has a mass of approximately 110 kDa. Researchers found ADAMTS15 expression mainly in cartilage and testis highlighting its tissue-specific roles. Understanding its mechanical role as a proteoglycanase gives insight into its contribution to tissue remodeling dynamics.
Biological function summary

ADAMTS15 plays a significant role in extracellular matrix organization and turnover. It mediates proteolytic processing of aggrecan and other proteoglycans which are important for structural integrity in tissues like cartilage. It operates independently rather than as part of a complex. The proteolytic function of ADAMTS15 is essential in processes like embryonic development cell migration and angiogenesis emphasizing its influence in maintaining normal physiological functions.

Pathways

ADAMTS15 is implicated in the proteoglycan turnover pathway and the Wnt signaling pathway. Its activities within these pathways contribute to cellular matrix degradation and regulation of cell growth and differentiation. ADAMTS15 associates with other proteins such as aggrecan and collagen that participate in matrix degradation. It shows functional similarities and interactions with other ADAMTS family members like ADAMTS4 and ADAMTS5 which further support its role in matrix dynamics.

Researchers link ADAMTS15 with osteoarthritis and some forms of cancer. In osteoarthritis abnormal activity of ADAMTS15 leads to excessive degradation of cartilage matrix contributing to joint degeneration. Proteins like aggrecan targeted by ADAMTS15 play a role in this disorder. In cancer ADAMTS15 involvement appears in the regulation of tumor microenvironment dynamics and metastasis. Its interaction with structural components of the matrix makes it a potential therapeutic target in both contexts.
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Product protocols

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Target data

Metalloprotease which has proteolytic activity against the proteoglycan VCAN, cleaving it at the 'Glu-1428-|-1429-Ala' site. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.
See full target information ADAMTS15
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB45047

Anti-ADAMTS15 antibody

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Proteins & Peptides

AB165819

Recombinant Human ADAMTS15 protein

SDS-PAGE - Recombinant Human ADAMTS15 protein (AB165819)

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