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AB108706

Human Anti-Adenovirus IgM ELISA Kit

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(1 Publication)

Human Anti-Adenovirus IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Adenovirus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Adenovirus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Adenovirus in Human serum and plasma.

A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108706-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Adenovirus anti-IgM HRP Conjugate", "number":"AB108706-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108706-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Adenovirus IgM Positive Control", "number":"AB108706-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"Adenovirus IgM Cut-off Control", "number":"AB108706-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Adenovirus IgM Negative Control", "number":"AB108706-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108706-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108706-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108706-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"1 Strip holder", "number":"AB108706-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adenovirus (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108706-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108706-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenovirus IgM is an antibody with a molecular weight of approximately 900 kDa. It is mainly expressed in the blood during the acute phase of adenovirus infections. Known for its pentameric structure Adenovirus IgM plays an important role in early immune response by targeting adenovirus capsid proteins. It forms part of the larger immunoglobulin family and marks the initial phase of serological detection following viral exposure.
Biological function summary

This immunoglobulin marks recent adenovirus infection and initial immune activation. During infections the immune system produces Adenovirus IgM which binds to the viral antigen hindering viral replication and promoting viral clearance. Whilst not part of large protein complexes it interacts directly with adenovirus structural proteins. This interaction helps in activating the complement system and facilitating phagocytosis by immune cells.

Pathways

The immune response against adenovirus involves the classical complement pathway and antibody-mediated antigen recognition. Adenovirus IgM initiates the complement cascade leading to opsonization and lysis of virus particles. It shares its function with Adenovirus IgG as both counter the viral infection. However IgM functions earlier than IgG during the infection timeline. The antibody's activity is important for the swift clearance of the virus.

Adenovirus can cause acute respiratory diseases and conjunctivitis. Adenovirus IgM serves as an indicator to diagnose these conditions in their early stages. Its presence signals the body’s initial immune response to these infections. The detection of IgM also connects with elevated levels of other acute phase proteins like C-reactive protein (CRP) which serve as markers of inflammation during infection.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 8:e56708 PubMed23516396

2013

Identification of a nonstructural DNA-binding protein (DBP) as an antigen with diagnostic potential for human adenovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Li Guo,Chengjun Wu,Hongli Zhou,Chao Wu,Gláucia Paranhos-Baccalà,Guy Vernet,Qi Jin,Jianwei Wang,Tao Hung
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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