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AB108786

Human Adiponectin ELISA Kit

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(7 Publications)

Human Adiponectin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Adiponectin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

ACDC, ACRP30, APM1, GBP28, ADIPOQ, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, apM-1

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Sandwich ELISA - Human Adiponectin ELISA Kit (AB108786)
  • sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB108786)

Representative Standard Curve using ab108786

Sandwich ELISA - Human Adiponectin ELISA Kit (AB108786)
  • sELISA

Unknown

Sandwich ELISA - Human Adiponectin ELISA Kit (AB108786)

Representative Standard Curve using ab108786

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.24 ng/mL

Range

0.391 - 25 ng/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Adiponectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Adiponectin levels in urine, plasma, serum, and milk, saliva, and cell culture supernatants. (UniprotID: Q15848)

An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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Product protocols

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Target data

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
See full target information ADIPOQ
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36674598

2023

Defining the Molecular Mechanisms of the Relaxant Action of Adiponectin on Murine Gastric Fundus Smooth Muscle: Potential Translational Perspectives on Eating Disorder Management.

Applications

Unspecified application

Species

Unspecified reactive species

Rachele Garella,Emanuele Cassioli,Flaminia Chellini,Alessia Tani,Eleonora Rossi,Eglantina Idrizaj,Daniele Guasti,Paolo Comeglio,Francesco Palmieri,Martina Parigi,Linda Vignozzi,Maria Caterina Baccari,Valdo Ricca,Chiara Sassoli,Giovanni Castellini,Roberta Squecco

Frontiers in pediatrics 10:969372 PubMed36120658

2022

Determination of salivary concentrations of leptin and adiponectin, ability to reduce ferric ions and total antioxidant capacity of saliva in patients with severe early childhood caries.

Applications

Unspecified application

Species

Unspecified reactive species

Bojan Petrović,Nebojsa Stilinović,Ana Tomas,Sanja Kojić,Goran M Stojanović

Frontiers in physiology 13:827335 PubMed35264977

2022

Effects of Three Different Modes of Resistance Training on Appetite Hormones in Males With Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Ataeinosrat,Marjan Mosalman Haghighi,Hossein Abednatanzi,Mohammad Soltani,Abbass Ghanbari-Niaki,Akbar Nouri-Habashi,Sadegh Amani-Shalamzari,Ali Mossayebi,Mitra Khademosharie,Kelly E Johnson,Trisha A VanDusseldorp,Ayoub Saeidi,Hassane Zouhal

Frontiers in endocrinology 11:177 PubMed32300333

2020

Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Song,Juanjuan Zou,Zhiyuan Song,Huajun Xu,Yinjun Qian,Huaming Zhu,Suru Liu,Jian Guan,Jie Chen,Hongliang Yi

Experimental and therapeutic medicine 19:2690-2696 PubMed32256750

2020

Icariin treatment reduces blood glucose levels in type 2 diabetic rats and protects pancreatic function.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Yun-Xiao Wang,Ping Shi,Yan-Ping Liu,Ting Li,Shu-Qin Liu,Chen-Jing Wang,Le-Xin Wang,Yu Cao

High altitude medicine & biology 20:165-170 PubMed31161940

2019

Effects of Hypobaric Hypoxia on Endothelial Function and Adiponectin Levels in Airforce Aviators.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Grittani,Grazia Pellegrino,Stefano Conte,Andrea Morello,Alberto Autore,Giovanni Cimmino,Bruno Trimarco,Fabio Morgagni,Plinio Cirillo

Journal of Cancer 7:1340-6 PubMed27390609

2016

Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongkai Wang,Junlong Wu,Weijie Gu,Beihe Wang,Fangning Wan,Bo Dai,Hailiang Zhang,Guohai Shi,Yijun Shen,Yiping Zhu,Yao Zhu,Dingwei Ye
View all publications
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