AB314604

Human Adiponectin ELISA Kit

SimpleStep
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Human Adiponectin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Adiponectin with a sensitivity of 5.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ACDC, ACRP30, APM1, GBP28, ADIPOQ, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, apM-1

6 Images

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Standard curve comparison between the original Human Adiponectin SimpleStep ELISA (ab222508) and current Human Adiponectin SimpleStep ELISA (ab314604).

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Example of human Adiponectin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Serum of ten male individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration for male serum samples was determined to be 4,564 ng/mL with a range of 2,229 – 6,751 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Standard curve comparison between Human Adiponectin SimpleStep ELISA kit (ab314604) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Serum of eight female individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Adiponectin concentration for female serum samples was determined to be 7,467 ng/mL with a range of 3,452 – 14,981 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 3,333 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.39 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Adiponectin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Adiponectin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant.Quantitate Human Adiponectin with 5.39 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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Recovery

[ { "sample": "Serum", "range": "98 - 113 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "101 - 117 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "92 - 115 %", "average": "= 104" }, { "sample": "Cell culture supernatant", "range": "100 - 110 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314604-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314604-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314604-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314604-CMP07", "productcode":"" }, { "size":"1 x 100 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314604-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB314604-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Adiponectin Capture Antibody", "number":"AB314604-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Adiponectin Lyophilized Recombinant Protein", "number":"AB314604-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Adiponectin Detector Antibody", "number":"AB314604-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314604-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314604-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.

Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.

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Product protocols

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Target data

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

See full target information ADIPOQ

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Adiponectin ELISA Kit

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Sandwich ELISA - Human Adiponectin ELISA Kit (AB314604)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

Product promise