Human Afamin (AFM) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Afamin (AFM) with a sensitivity of 13.47 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 70.313 - 4500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13.47 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed:26902720). Binds vitamin E (PubMed:12463752, PubMed:15952736). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed:15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed:19046407).
Alternative names
ALB2, ALBA, AFM, Afamin, Alpha-albumin, Alpha-Alb
What's included?
Human Afamin (AFM) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Afamin (AFM) with a sensitivity of 13.47 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 70.313 - 4500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 13.47 pg/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96 | Range 89 - 101 % |
Sample type Citrate plasma | Average % = 97 | Range 92 - 103 % |
Sample type EDTA Plasma | Average % = 100 | Range 99 - 102 % |
Sample type Heparin Plasma | Average % = 95 | Range 85 - 102 % |
Sample type Cell culture supernatant | Average % = 102 | Range 100 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Afamin (AFM) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Afamin (AFM) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Afamin (AFM) with 13.47 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Interpolated concentrations of human Afamin in serum in 96-well vs. 384-well plates.
Interpolated concentration of native Afamin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:20,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Example of human Afamin standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate.
Example of human Afamin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Interpolated concentrations of Afamin in normal human serum donors.
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Afamin concentration was determined to be 48.82 µg/mL with a range of 35.29 – 73.71 µg/mL.
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Interpolated concentrations of human Afamin in HepG2 supernatant.
Interpolated concentration of native Afamin was measured in duplicate at different sample concentrations. Undiluted samples are 100% HepG2 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Interpolated concentrations of human Afamin in serum and plasma.
Interpolated concentration of native Afamin was measured in duplicate at different sample concentrations. Undiluted samples are 1:20,000 serum, plasma (citrate), plasma (EDTA), ad plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Afamin (AFM) ELISA Kit (ab323527)
Example of human Afamin standard curve in Sample Diluent NS + 1X Enhancer.
Example of human Afamin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
- Visit the general protocols
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