AB240687

Human AG-2 ELISA Kit

Recombinant
SimpleStep
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Human AG-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AG-2 with a sensitivity of 10.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

AG2, UNQ515/PRO1030, AGR2, Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog

3 Images

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

sELISA

Supplier Data

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Example of human AG-2 standard curve in 1X Cell Extraction Buffer PTR prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Representative image of Human AG-2 ELISA Kit (ab240687)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human AG-2 ELISA Kit Capture Antibody

- 10X Human AG-2 Detector Antibody

- 10X Human AG-2 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human AG-2 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Interpolated concentrations of native AG-2 in human PC3 cell extract based on a 1.3 µg/mL extract load and in human fetal small intestine tissue homogenate extract based on a 12.5 µg/mL extract load. The concentrations of AG-2 were measured in duplicate and interpolated from the AG-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AG-2 concentration was determined to be 827 pg/mL in PC3 cell extract and 547 pg/mL in human fetal small intestine tissue homogenate extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.6 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human AG-2 ELISA Kit (ab240687) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AG-2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AG-2 with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 8", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": null, "average": "= 97" }, { "sample": "Tissue Extracts", "range": null, "average": "= 99" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab240687-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240687-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB240687-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240687-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB240687-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB240687-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB240687-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240687-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AG-2 Capture Antibody", "number":"AB240687-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB240687-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AG-2 Lyophilized Recombinant Protein", "number":"AB240687-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB240687-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human AG-2 Detector Antibody", "number":"AB240687-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anterior Gradient 2 (AG2) also known as AGR2 is a protein with a molecular weight of approximately 20 kDa. This protein is a member of the protein disulfide isomerase family and has an important mechanical role in facilitating protein folding within the endoplasmic reticulum. The protein exists mainly in epithelial tissues and notably in the gastrointestinal tract with high expression also found in the pancreas and lungs. AG2 is characterized by its interaction with various client proteins assisting in their proper folding and stability.

Biological function summary

The anterior gradient protein influences cellular processes such as proliferation and differentiation often playing a role in cellular adhesion and migration. This protein does not work alone but instead can form complexes with other molecules enhancing its functional capabilities. Such interactions highlight its significance in maintaining normal cell physiology and response to external stimuli.

Pathways

Anterior gradient protein participates in key signaling pathways including the Wnt signaling pathway and the unfolded protein response (UPR). Through these pathways AG2 associates with proteins like β-catenin influencing gene transcription and cellular outcomes. In the UPR its role is important in managing cellular stress and maintaining homeostasis by regulating protein folding and quality control mechanisms.

Anterior gradient protein shows strong associations with cancer particularly in tumors such as breast and lung cancer where it is often overexpressed. Its involvement links closely with proteins like MUC1 which is found in tumors suggesting a role in promoting cancer progression and metastasis. Additionally AG2 can be related to inflammatory bowel diseases where its dysregulation may result in epithelial barrier dysfunction and contribute to the pathology.

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Product protocols

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Target data

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed : 23274113).

See full target information AGR2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human AG-2 ELISA Kit

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Sandwich ELISA - Human AG-2 ELISA Kit (AB240687)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise