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AB216945

Human Agrin ELISA Kit

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(1 Review)

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(17 Publications)

Human Agrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Agrin with a sensitivity of 4.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

AGRIN, AGRN, Agrin

8 Images
ELISA - Human Agrin ELISA Kit (AB216945)
  • ELISA

Supplier Data

ELISA - Human Agrin ELISA Kit (AB216945)
Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Agrin concentration in neat samples was determined to be 3909 pg/mL with a range of 2879 – 5073 pg/mL.

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Interpolated concentrations of native Agrin in human brain tissue extract, human liver tissue extract, SH-SY5Y cell extract and HepG2 cell extract.

Interpolated concentrations of native Agrin in human brain tissue extract based on a 300 μg/mL extract load, human liver tissue extract based on a 1000 μg/mL extract load, SH-SY5Y cell extract based on a 500 μg/mL extract load, and HepG2 cell extract based on a 100 μg/mL extract load. The concentrations of Agrin were measured in duplicate and interpolated from the Agrin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Agrin concentration was determined to be 540 pg/mL in brain tissue extract, 740 pg/mL in liver tissue extract, 252 pg/mL in SH-SY5Y cell extract, and 796 pg/mL in HepG2 cell extract.

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Interpolated concentrations of native Agrin in human urine, cerebrospinal fluid, and cell culture supernatant samples.

The concentrations of Agrin were measured in duplicates, interpolated from the Agrin standard curves and corrected for sample dilution. Undiluted samples are as follows : Urine 12.5%, cerebrospinal fluid 6.25%, HepG2 cell culture supernatant 100% and HeLa cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Agrin concentration was determined to be 9,142 pg/mL in neat urine, 18,572 pg/mL in neat cerebrospinal fluid, 1,365 pg/mL in HepG2 cell culture supernatant, and 711.7 pg/mL in HeLa cell culture supernatant.

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Example of human Agrin standard curve in HGDMEM + 10% FBS cell culture media.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Interpolated concentrations of native Agrin in human serum and plasma samples.

The concentrations of Agrin were measured in duplicates, interpolated from the Agrin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Agrin concentration was determined to be 3,719 pg/mL in neat serum, 3,562 pg/mL in neat plasma (citrate), 3,526 pg/mL in neat plasma (heparin), and 3,445 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Example of human Agrin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)
  • sELISA

Supplier Data

Sandwich ELISA - Human Agrin ELISA Kit (AB216945)

Example of human Agrin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.6 pg/mL

Range

28.13 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB242996

Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector) (AB242996)

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Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Agrin ELISA Kit ab216945 is a rapid single-wash 90-min Sandwich ELISA to measure Human Agrin in cell culture extracts, cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 4.6 pg/mL.

How the assay works

Human Agrin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Agrin ELISA Kit ab216945 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Agrin antibody [EPR20025-20] - BSA and Azide free (Capture) ab242996
- Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector) ab242996

Agrin is a heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Agrin is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. Agrin function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Agrin modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Agrin functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "116 - 121 %", "average": "= 119" }, { "sample": "Serum", "range": "87 - 113 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "104 - 109 %", "average": "= 106" }, { "sample": "Cerebral Spinal Fluid", "range": "86 - 126 %", "average": "= 106" }, { "sample": "Urine", "range": "101 - 114 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "90 - 103 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "115 - 122 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "101 - 112 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "97 - 121 %", "average": "= 113" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Agrin also known as AGRN is a large heparan sulfate proteoglycan with a molecular mass of approximately 200 kDa. This protein is mainly expressed in the neuromuscular junction and the central nervous system. Agrin has significant roles in synapse formation. It influences the clustering of acetylcholine receptors at the neuromuscular junction which is essential for communication between nerve and muscle cells. This activity is mechanically mediated through interactions with the receptor tyrosine kinase MuSK and LRP4.
Biological function summary

The Agrin protein contributes to the stabilization of synaptic structures. Its ability to induce acetylcholine receptor clustering is fundamental for the proper formation and maintenance of neuromuscular synapses. Agrin is not a lone player; it is a part of a larger signaling complex. This complex includes other proteins like MuSK which acts as a receptor playing an essential role in complex signaling pathways that regulate synaptogenesis and synaptic plasticity.

Pathways

Many molecules associate to facilitate communication and synapse integrity via Agrin's activity. Agrin engages primarily with the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are central for cell growth and differentiation in the central nervous system. The relationship with MuSK through these pathways highlights Agrin's importance in orchestrating the synapse assembly and stability.

Mutations or alterations in Agrin can lead to conditions such as congenital myasthenic syndromes and potentially impact neurodegenerative disorders like amyotrophic lateral sclerosis. In these cases its interaction with proteins like MuSK is affected leading to impaired neuromuscular transmission. Understanding the anti-Agrin antibody's function might enhance methodologies to treat these neuromuscular disorders.
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Product protocols

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Target data

Depending on alternative splicing and post-translational modifications, it has a role in different processes, including neuromuscular junction formation and maintenance, and regulation of neurite outgrowth (By similarity). Also involved in positive regulation of cartilage formation through alpha-dystroglycan binding and up-regulation of SOX9 (PubMed : 26290588).. Isoform 1. Heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK (PubMed : 21969364). The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This secreted isoform forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the NtA domain.. Isoform 2. Transmembrane form that is the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.. Isoform 1, isoform 4 and isoform 5 : neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.. Isoform 3. Muscle-specific isoform, probably involved in endothelial cell differentiation.. Isoform 6. Involved in the positive regulation of cartilage formation, acting through alpha-dystroglycan binding and up-regulation of SOX9, a transcription factor that plays a key role in chondrocytes differentiation.. Agrin N-terminal 110 kDa subunit. Is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling (By similarity).. Agrin C-terminal 22 kDa fragment. This released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
See full target information AGRN
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 16:e13832 PubMed40464208

2025

Serum C-Terminal Agrin Fragment With Acute and Long-Term Exercise and Angiotensin II Type I Receptor Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Casper Soendenbroe,Mette F Heisterberg,Jesper L Andersen,Michael Kjaer,Abigail L Mackey

Journal of cachexia, sarcopenia and muscle 16:e13707 PubMed39887577

2025

C-Terminal Agrin Fragment as a Biomarker for Sarcopenia: A Systematic Review and Meta-Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Rida Fatima,Yonghoon Kim,Suhyeon Baek,Reema Priyanka Suram,Sun-Joung Leigh An,Yonggeun Hong

Aging cell 24:e14413 PubMed39555723

2024

Muscle fibroblasts and stem cells stimulate motor neurons in an age and exercise-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Casper Soendenbroe,Peter Schjerling,Cecilie J L Bechshøft,Rene B Svensson,Laurent Schaeffer,Michael Kjaer,Bénédicte Chazaud,Arnaud Jacquier,Abigail L Mackey

PLoS pathogens 20:e1012547 PubMed39312589

2024

HIV reservoir and premature aging: risk factors for aging-associated illnesses in adolescents and young adults with perinatally acquired HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Raffaella Petrara,Elena Ruffoni,Francesco Carmona,Ilaria Cavallari,Sandra Zampieri,Marzia Morello,Paola Del Bianco,Osvalda Rampon,Nicola Cotugno,Paolo Palma,Paolo Rossi,Carlo Giaquinto,Silvia Giunco,Anita De Rossi

Journal of cachexia, sarcopenia and muscle 15:1501-1510 PubMed38845597

2024

Plasma C-terminal agrin fragment concentrations across adulthood: Reference values and associations with skeletal muscle health.

Applications

Unspecified application

Species

Unspecified reactive species

Jedd Pratt,Evgeniia Motanova,Ludmilla Pessanha,Marco Narici,Colin Boreham,Giuseppe De Vito

Skeletal muscle 14:5 PubMed38454511

2024

Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Suradip Das,Melanie C Hilman,Feikun Yang,Foteini Mourkioti,Wenli Yang,D Kacy Cullen

Aging clinical and experimental research 35:2563-2571 PubMed37658983

2023

Handgrip strength asymmetry as a new biomarker for sarcopenia and individual sarcopenia signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Jedd Pratt,Ludmilla Pessanha,Marco Narici,Colin Boreham,Giuseppe De Vito

Aging clinical and experimental research 35:1161-1186 PubMed36977974

2023

Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review.

Applications

Unspecified application

Species

Unspecified reactive species

Prabal Kumar,Kusumakshi Nayak,Shashikiran Umakanth,N Girish

Frontiers in medicine 10:1094733 PubMed36891188

2023

Serum transthyretin and aminotransferases are associated with lean mass in people with coronary heart disease: Further insights from the CARE-CR study.

Applications

Unspecified application

Species

Unspecified reactive species

Emily James,Stuart Goodall,Simon Nichols,Karen Walker,Sean Carroll,Alasdair F O'Doherty,Lee Ingle

Journal of cachexia, sarcopenia and muscle 14:794-804 PubMed36708273

2023

Effects of a 2-year exercise training on neuromuscular system health in older individuals with low muscle function.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Monti,Sara Tagliaferri,Sandra Zampieri,Fabio Sarto,Giuseppe Sirago,Martino Vladimiro Franchi,Andrea Ticinesi,Yari Longobucco,Elisa Adorni,Fulvio Lauretani,Stephan Von Haehling,Emanuele Marzetti,Riccardo Calvani,Roberto Bernabei,Matteo Cesari,Marcello Maggio,Marco Vincenzo Narici
View all publications
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