Human AGT ELISA Kit ab287170 is a single-wash 90-min SimpleStep ELISA used to quantify Human AGT with a sensitivity of 26.54 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human AGT sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.125 - 5000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 26.54 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed:10619573, PubMed:17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed:1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity). Angiotensin-3. Stimulates aldosterone release. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
Alternative names
SERPINA8, AGT, Angiotensinogen, Serpin A8
What's included?
Recommended products
Human AGT ELISA Kit ab287170 is a single-wash 90-min SimpleStep ELISA used to quantify Human AGT with a sensitivity of 26.54 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human AGT sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.125 - 5000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 26.54 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 107 | Range 104 - 110 % |
Sample type EDTA Plasma | Average % = 104 | Range 98 - 109 % |
Sample type Cell culture media | Average % = 97 | Range 83 - 110 % |
Sample type Heparin Plasma | Average % = 105 | Range 91 - 117 % |
Sample type Citrate plasma | Average % = 88 | Range 85 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human AGT ELISA kit (ab287170) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human AGT protein in human serum, plasma, cell culture supernatant and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AGT with 26.54 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.
- Biological function summary
Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.
- Pathways
Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.
- Associated diseases and disorders
Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.
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5 product images
Sandwich ELISA - Human AGT ELISA Kit (ab287170)
Example of human AGT standard curve in Sample Diluent NS.
Example of human AGT standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AGT ELISA Kit (ab287170)
Example of human AGT standard curve in 1X Cell Extraction Buffer PTR.
Example of human AGT standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AGT ELISA Kit (ab287170)
Interpolated concentrations of human AGT in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 1:20,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human AGT ELISA Kit (ab287170)
Interpolated concentrations of human AGT in human lung tissue extract.
Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 25 μg/mL human lung tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human AGT ELISA Kit (ab287170)
Interpolated concentrations of AGT in normal human serum donors.
1:20,000 serum of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean AGT concentration was determined to be 66.70 μg/mL with a range of 40.19 – 194.33 μg/mL.
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