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AB287170

Human AGT ELISA Kit

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Human AGT ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AGT with a sensitivity of 26.54 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SERPINA8, AGT, Angiotensinogen, Serpin A8

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ELISA - Human AGT ELISA Kit (AB287170)
  • ELISA

Supplier Data

ELISA - Human AGT ELISA Kit (AB287170)
Sandwich ELISA - Human AGT ELISA Kit (AB287170)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit (AB287170)

Interpolated concentrations of AGT in normal human serum donors.

1 : 20,000 serum of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean AGT concentration was determined to be 66.70 μg/mL with a range of 40.19 – 194.33 μg/mL.

Sandwich ELISA - Human AGT ELISA Kit (AB287170)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit (AB287170)

Interpolated concentrations of human AGT in human lung tissue extract.

Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 25 μg/mL human lung tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human AGT ELISA Kit (AB287170)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit (AB287170)

Interpolated concentrations of human AGT in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human AGT ELISA Kit (AB287170)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit (AB287170)

Example of human AGT standard curve in Sample Diluent NS.

Example of human AGT standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AGT ELISA Kit (AB287170)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit (AB287170)

Example of human AGT standard curve in 1X Cell Extraction Buffer PTR.

Example of human AGT standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 26.54 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AGT ELISA Kit ab287170 is a rapid single-wash 90-min sandwich ELISA kit to measure Human AGT in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture media, and Tissue Extracts. This Simplestep sensitivity is 26.54 pg/mL.

How the assay works

Human AGT SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Human AGT ELISA Kit ab287170 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Angiotensinogen antibody [EPR24118-30] - BSA and Azide free (Capture) ab276023
- Anti-Angiotensinogen antibody [EPR24118-74] - BSA and Azide free (Detector) ab276024

Related and Recommended products

Human AGT ELISA Kit ab287170 is commonly used to measure Angiotensinogen (AGT) as a part of the renin-angiotensin system (RAS) in hypertension and cardiovasular diseases.

Other ELISA kits to measure RAS markers include:

- Human Renin ELISA Kit ab246545
- Human ACE ELISA Kit ab263889
- Human ACE2 ELISA Kit ab235649

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 110 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "98 - 109 %", "average": "= 104" }, { "sample": "Cell culture media", "range": "83 - 110 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "91 - 117 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287170-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287170-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287170-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287170-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287170-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287170-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287170-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB287170-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AGT Lyophilized Recombinant Protein", "number":"AB287170-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AGT Capture Antibody", "number":"AB287170-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AGT Detector Antibody", "number":"AB287170-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB287170-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.
Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed : 10619573, PubMed : 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed : 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
See full target information AGT
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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