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AB325899

Human AGT ELISA Kit, Chemiluminescent

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Human AGT ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human AGT with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 49.38 - 12000 pg/ml with a sensitivity of 19.3 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

SERPINA8, AGT, Angiotensinogen, Serpin A8

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Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Lab

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Lab

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Dynamic range comparison between the Chemiluminescent Human AGT SimpleStep ELISA, colorimetric Human AGT SimpleStep ELISA (ab287170), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased dynamic range.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Lab

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Standard curve comparison between the Chemiluminescent Human AGT SimpleStep ELISA and colorimetric Human AGT SimpleStep ELISA (ab287170) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Lab

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Standard curve comparison between the Chemiluminescent Human AGT SimpleStep ELISA and colorimetric Human AGT SimpleStep ELISA (ab287170) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Interpolated concentrations of AGT in normal human serum donors. 1 : 20,000 serum of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AGT concentration was determined to be 66.70 μg/mL with a range of 40.19 – 194.33 μg/mL.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 25 μg/mL human lung tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)
  • sELISA

Supplier Data

Sandwich ELISA - Human AGT ELISA Kit, Chemiluminescent (AB325899)

Interpolated concentration of native AGT was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 19.3 pg/mL

Range

49.38 - 12000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human AGT ELISA Kit, Chemiluminescent (ab325899) is a rapid single-wash sandwich 55-min ELISA to measure Human AGT in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media, Tissue Extract with a sensitivity of 19.3 pg/mL.

How the assay works

Human AGT ELISA Kit, Chemiluminescent (ab325899) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human AGT (ab325899) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-AGT antibody [EPR24118-30] - BSA and Aside free (Capture) ab276023
- Anti-AGT antibody [EPR24118-74] - BSA and Aside free (Detector) ab276024

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 110 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "98 - 109 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "91 - 117 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "83 - 110 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "84 - 91 %", "average": "= 88" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed : 10619573, PubMed : 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed : 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
See full target information AGT
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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