AB184858

Human AIF ELISA Kit (Apoptosis-Inducing Factor 1)

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) is a single-wash 90-min Simplestep used to quantify Human AIF (Apoptosis-Inducing Factor 1) with a sensitivity of 60 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

AIF, PDCD8, AIFM1, Programmed cell death protein 8

3 Images

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

sELISA

Unknown

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Example of human AIF standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Interpolated concentrations of native AIF in human HeLa cell extract based on a 66.6 μg/mL extract load and human heart tissue extract based on a 20 μg/mL extract load.

The concentrations of AIF were measured in duplicate and interpolated from the AIF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AIF concentration was determined to be 4.106 ng/mL in HeLa cell extract and 9.364 ng/mL in human heart tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Representative image of Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (ab184858)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human AIF (Apoptosis-Inducing Factor 1) Detector Antibody

- Human AIF (Apoptosis-Inducing Factor 1) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human AIF (Apoptosis-Inducing Factor 1) Capture Antibody (Lyophilised) (2 vials)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CPI2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 60 pg/mL

Range

0.156 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human AIF (Apoptosis-Inducing Factor 1) ELISA kit (ab184858) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AIF protein in human serum, plasma, cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human AIF with 60 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

AIF functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, AIF functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form of AIF (AIFsol) is found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. AIF interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. AIF plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. AIF binds to DNA in a sequence-independent manner.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "102 - 105.6 %", "average": "= 103.2" }, { "sample": "Cell culture media", "range": "97.3 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "101.7 - 110 %", "average": "= 106.2" }, { "sample": "EDTA Plasma", "range": "104.1 - 107.3 %", "average": "= 105.3" }, { "sample": "Heparin Plasma", "range": "105.6 - 108.4 %", "average": "= 107" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab184858-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB184858-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB184858-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB184858-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB184858-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB184858-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB184858-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AIF Detector Antibody", "number":"AB184858-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AIF Lyophilized Recombinant Protein", "number":"AB184858-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AIF Capture Antibody (Lyophilized)", "number":"AB184858-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB184858-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB184858-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB184858-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIF short for Apoptosis-Inducing Factor is a flavoprotein characterized by its mass of approximately 57 kDa. It resides in the mitochondria of cells where it plays a decisive role in apoptosis. Under normal conditions AIF remains in the mitochondrial intermembrane space but when cells receive apoptotic signals AIF translocates to the nucleus leading to chromatin condensation and large-scale DNA fragmentation. The protein is also known as AIFM1 and performs functions beyond apoptosis including regulation of reactive oxygen species. Often analyzed using tools like a mitochondrial AB marker researchers use AIF as an important part of understanding cell death mechanisms.

Biological function summary

AIF functions as an important element in mitochondrial-mediated apoptosis. Once released from mitochondria AIF triggers a caspase-independent pathway of apoptosis making it distinct from other pathways where caspases are central. While not part of a larger protein complex AIF interacts closely with several mitochondrial and nuclear elements to execute its functions. Researchers often monitor AIF activity using apoptosis ELISA kits which help in precise detection and quantification of this protein within cellular systems.

Pathways

The presence and activity of AIF align with the broader apoptotic pathway and mitochondrial respiration. AIF links significantly with other molecules like cytochrome c released during apoptosis although cytochrome c follows a caspase-dependent pathway. It also shows involvement in various cellular injury and stress response pathways reinforcing its role as a major player in cellular fate decisions and intrinsic apoptotic mechanisms.

AIF holds relevance in neurodegenerative disorders such as Parkinson's disease and certain forms of cancer. Misregulation or mutations in AIF can lead to increased susceptibility to these conditions highlighting its importance in maintaining normal cellular and mitochondrial function. AIF also interacts with other proteins implicated in these disorders such as E2F1 in cancer which shares similar apoptotic regulatory roles. These interactions highlight the biological significance of AIF beyond its fundamental apoptosis function making it a potential target for therapeutic interventions.

style

left-column

Product protocols

style

left-column

Target data

Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed : 17094969, PubMed : 20362274, PubMed : 23217327, PubMed : 33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed : 20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed : 20362274). Binds to DNA in a sequence-independent manner (PubMed : 27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed : 17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed : 19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed : 26004228).. Isoform 4. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.. Isoform 5. Pro-apoptotic isoform.

See full target information AIFM1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human AIF ELISA Kit (Apoptosis-Inducing Factor 1)

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Sandwich ELISA - Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (AB184858)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Product promise