AB254506

Human AKR1B10 ELISA Kit

Recombinant
SimpleStep
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Human AKR1B10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AKR1B10 with a sensitivity of 10.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

AKR1B11, AKR1B10, Aldo-keto reductase family 1 member B10, ARL-1, Aldose reductase-like, Aldose reductase-related protein, Small intestine reductase, ARP, hARP, SI reductase

3 Images

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

sELISA

Supplier Data

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Representative image of Human AKR1B10 ELISA Kit (ab254506)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human AKR1B10 Capture Antibody

- 10X Human AKR1B10 Detector Antibody

- Human AKR1B10 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Interpolated concentrations of native AKR1B10 in human A549 cell extract based on a 0.31 μg/mL extract load.

The concentrations of AKR1B10 were measured in duplicate and interpolated from the AKR1B10 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Example of human AKR1B10 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The AKR1B10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10.3 pg/mL

Range

78 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human AKR1B10 ELISA Kit (ab254506) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AKR1B10 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AKR1B10 with 10.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Aldo-Keto Reductase Family-1 member B10 (AKR1B10), also referred to as Aldose Reductase-like protein, is an 316- amino acid enzyme encoded by the AKR1B10 gene. AKR1B10 belongs to the aldo/keto reductase superfamily, where AKR1B10’s function is to catalyze the NADPH-dependent reduction of carbonyls to the corresponding alcohols. Found to be highly expressed in the small intestine and colon, AKR1B10 is thought to be responsible in detoxifying dietary and lipid derived carbonyls and may play a role in liver carcinogenesis. AKR1B10 has no counterpart in mouse or rat species.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "106 - 120 %", "average": "= 112" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254506-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254506-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254506-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB254506-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254506-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254506-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB254506-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB254506-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AKR1B10 Lyophilized Recombinant Protein", "number":"AB254506-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AKR1B10 Capture Antibody", "number":"AB254506-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AKR1B10 Detector Antibody", "number":"AB254506-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254506-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB254506-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldo-keto reductase family 1 member B10 (AKR1B10) also known as ARL-1 (aldo-keto reductase-like-1) is an enzyme encoded by the AKR1B10 gene. The enzyme has a molecular weight of approximately 36 kDa. It is mainly expressed in the liver gastrointestinal tract and to a lesser extent in the lung and adrenal gland. As an enzyme AKR1B10 catalyzes the reduction of carbonyl groups to their corresponding alcohols using NADPH as a cofactor. This function plays a role in the detoxification of aldehydes and ketones.

Biological function summary

AKR1B10 protects cells from disorders caused by toxic aldehydes. It is important in lipid biosynthesis through the reduction of retinaldehyde to retinol which is part of the retinoic acid metabolism. AKR1B10 forms part of the aldo-keto reductase superfamily consisting of multiple related enzymes that regulate various cellular processes. This enzyme class is involved in stabilizing the oxidoreduction balance within cells.

Pathways

AKR1B10 engages in lipid metabolism and the protection against oxidative stress. It is an important player in the retinoid metabolism and signaling pathway where it collaborates with enzymes like retinaldehyde dehydrogenase. Additionally AKR1B10 is involved in the polyol pathway although its exact role here continues to be studied. The enzyme's relationship with NADPH suggests it could intersect with systems managing cellular responses to oxidative stress.

AKR1B10 is associated with certain cancers and complications related to chronic alcohol consumption. Overexpression of AKR1B10 has been linked to hepatocellular carcinoma where it possibly collaborates with proteins like the carcinogenic beta-catenin pathway. Furthermore it plays a part in promoting resistance to chemotherapeutic drugs due to its detoxification role. In liver diseases caused by alcohol AKR1B10 helps protect hepatocytes from oxidative and aldehyde stress with aldehyde dehydrogenase being another involved enzyme in these processes.

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Product protocols

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Target data

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed : 12732097, PubMed : 18087047, PubMed : 19013440, PubMed : 19563777, PubMed : 9565553). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed : 12732097, PubMed : 18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed : 19013440, PubMed : 19563777). Displays no reductase activity towards glucose (PubMed : 12732097).

See full target information AKR1B10

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human AKR1B10 ELISA Kit

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Sandwich ELISA - Human AKR1B10 ELISA Kit (AB254506)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Proteins & Peptides

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Product promise