Human AKT3 (pS473) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AKT3 (pS473) with a sensitivity of 19.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 19.65 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
Human AKT3 (pS473) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AKT3 (pS473) with a sensitivity of 19.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 19.65 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 2.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 24 | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 97 | Range 94 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ATK (pS473) ELISA kit (ab270887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ATK (pS473) protein in human cell extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ATK (pS473) with 5.9 units/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
AKT3 is a member of the AKT family that includes AKT1 and AKT2. These serine/threonine-protein kinases are involved in diverse processes including glucose uptake, cell cycle progression, and apoptosis. AKT3 plays an important role in brain development and is crucial for glioma cells malignancy. Phosphorylation of Serine 473 is a known regulator of AKT’s kinase activity. The antibodies for this kit were raised against an AKT3-specific N-terminal fragment, as well as a phospho-peptide encompassing the 473-serine site, which shares high sequence identity for all three AKT members. The protein standard in this product is recombinant human AKT3 ligated with peptide containing phosphorylated Serine 416.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AKT3 also known as protein kinase B gamma plays an important role in various cellular processes. This serine/threonine kinase with a molecular mass of approximately 56 kDa functions as part of the AKT family which includes AKT1 and AKT2. AKT3 expresses highly in the brain tissues with significant expression also observed in the heart and kidneys. As an isoform of the AKT kinase it participates in signaling pathways essential for cell survival and metabolism.
- Biological function summary
AKT3 participates in regulating cell proliferation and survival. It operates as part of the PI3K/AKT signaling complex a central hub for signaling growth factors. This pathway is critical for transmitting signals from the cell surface to the nucleus affecting transcription and cell cycle progression. AKT3 protein specifically contributes significant roles in neuronal development and function due to its high expression within the nervous system.
- Pathways
AKT3 influences the PI3K/AKT and mTOR pathways. These pathways regulate cell growth metabolism and survival playing significant roles in normal cellular functions and cancer. AKT3 interacts closely with other signaling molecules including mTORC1 and mTORC2 complexes. While participating in these pathways AKT3 coordinates with the protein E17K which is pattern related with alterations leading to abnormal signaling and growth.
- Associated diseases and disorders
AKT3 is implicated in cancer and neurological disorders. Aberrations in its signaling can lead to tumorigenesis as seen in glioblastomas. Abnormal AKT3 activity associates with epilepsy and other neurodevelopmental disorders emphasizing its impact on neuronal functions. Connecting AKT3 with disorders the protein E17K mutation in AKT3 dysregulates normal kinase activity contributing to these pathologies.
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3 product images
Sandwich ELISA - Human AKT3 (pS473) ELISA Kit (ab270887)
Comparison of native AKT3 (pS473) concentration in treated versus control cell extracts. SH-SY5Y cells were treated with 10 µM pervanadate for 20 minutes, scraped and buffer exchanged into PBS + 20 mM NaF, followed by protein extraction. Mock control SH-SY5Y cells accompanied cell treatment. Concentrations of AKT3 (pS473) were measured in duplicate and interpolated against the standard curve. Results show the mean AKT3 (pS473) concentration in control cell extract was 30 pg/mL compared to 2,795 pg/mL in pervanadate treated samples.
Sandwich ELISA - Human AKT3 (pS473) ELISA Kit (ab270887)
Interpolated concentration of native AKT3 (pS473) was measured in duplicate at different sample concentrations. Undiluted samples are 0.5 mg/mL treated SH-SY5Y cell extract (10 µM pervanadate for 20 min). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human AKT3 (pS473) ELISA Kit (ab270887)
Example of human AKT3 (pS473) standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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