Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.
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- Biomedicines 9:2021Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung-Su Kim,Arun Asif,Abdul Rahim Chethikkattuveli Salih,Jae-Wook Lee,Ki-Nam Hyun,Kyung-Hyun ChoiPubMed 34680487
- Nano convergence 8:32021Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHafiz Muhammad Umer Farooqi,Bohye Kang,Muhammad Asad Ullah Khalid,Abdul Rahim Chethikkattuveli Salih,Kinam Hyun,Sung Hyuk Park,Dongeun Huh,Kyung Hyun ChoiPubMed 33528697
- The American journal of the medical sciences 350:374-92015Free 25-Hydroxyvitamin D Concentrations in Cystic Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoon Jeong Lee,Malcolm D Kearns,Ellen M Smith,Li Hao,Thomas R Ziegler,Jessica A Alvarez,Vin TangprichaPubMed 26512456
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 1.5 - 25 µg/mL
- Assay time
- 2h
- Sensitivity
- = 0.29 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
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Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 1.5 - 25 µg/mL
- Assay time
- 2h
- Assay Platform
- Microplate
- Sensitivity
- = 0.29 µg/mL
- General recovery
- 95%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean 5 % | SD - | C.V. - |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean 8.7 % | SD - | C.V. - |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Serum Albumin ELISA Kit (Human Albumin ELISA Kit ab179887) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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1 product image
Competitive ELISA - Human Albumin ELISA Kit (ab108787)
Representative Standard Curve using ab108787.
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Product protocols
- Visit the general protocols
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