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Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.

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Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Competitive

Reactive species

Human

Range

1.5 - 25 µg/mL

Assay time

2h

Sensitivity

= 0.28 µg/mL

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Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

What's included?

1 x 96 Tests
Components
100X Streptavidin-Peroxidase Conjugate
1 x 80 µL
10X Diluent N Concentrate
1 x 30 mL
1X Biotinylated Human Albumin (Lyophilized)
1 x 1 Vial
20X Wash Buffer Concentrate
1 x 30 mL
Albumin Microplate (12 x 8 well strips)
1 x 1 Unit
Albumin Standard
1 x 1 Vial
Chromogen Substrate
1 x 7 mL
Sealing Tapes
3 x 1 Unit
Stop Solution
1 x 11 mL

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Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Competitive

Reactive species

Human

Range

1.5 - 25 µg/mL

Assay time

2h

Assay Platform

Microplate

Sensitivity

= 0.28 µg/mL

General recovery

98%

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
3.3

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
10.6

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.

An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.

Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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