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Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.
Alternative names=Albumin, PRO2044, PRO1708, PRO0903, PRO2619, PRO2675, UNQ696/PRO1341, GIG42, GIG20, ALB
Colorimetric
Plasma, Serum
Competitive
Human
1.5 - 25 µg/mL
2h
= 0.28 µg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Human Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Human Albumin in Human in Plasma, Serum samples.
Alternative names=Albumin, PRO2044, PRO1708, PRO0903, PRO2619, PRO2675, UNQ696/PRO1341, GIG42, GIG20, ALB
Colorimetric
Plasma, Serum
Competitive
Human
1.5 - 25 µg/mL
2h
Microplate
= 0.28 µg/mL
98%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 3.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.6 |
Blue Ice
-20°C
Multi
Please refer to protocols
Abcam's Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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