Human Albumin ELISA kit is a sandwich ELISA used to quantify human albumin (ALB) with a sensitivity of 0.79 ng/mL
- Broad sample compatibility – works with urine, saliva, milk, cerebrospinal fluid (CSF), cell culture supernatants, cell lysates, and tissue samples
- Wide dynamic range – Detects albumin levels from 3.125–200 ng/mL
- Efficient assay process – Total assay time of approximately 3 hours
- Comprehensive kit components: Includes pre-coated 96-well microplate
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- Nature medicine 29:2866-28842023Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel K Wilton,Kevin Mastro,Molly D Heller,Frederick W Gergits,Carly Rose Willing,Jaclyn B Fahey,Arnaud Frouin,Anthony Daggett,Xiaofeng Gu,Yejin A Kim,Richard L M Faull,Suman Jayadev,Ted Yednock,X William Yang,Beth StevensPubMed 37814059
- Acta biochimica et biophysica Sinica 55:601-6122023Adipose-derived stem cells show hepatic differentiation potential and therapeutic effect in rats with acute liver failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinpeng Jin,Rongfeng Shi,Tangkai Qi,Qiang Li,Cong Chen,Shane Gao,Fengjuan Gao,Danjing Yang,Guangxi Sun,Jianqing Xu,Qingchun Fu,Jun Xu,Xiaoyan ZhangPubMed 37078751
- Science advances 9:eadf44902023IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Carbonaro,Kehui Wang,Hui Huang,Davor Frleta,Aditi Patel,Alexander Pennington,Mathieu Desclaux,Sven Moller-Tank,Justin Grindley,Judith Altarejos,Jun Zhong,Greg Polites,William Poueymirou,Stephen Jaspers,Christos Kyratsous,Brian Zambrowicz,Andrew Murphy,John C Lin,Lynn E Macdonald,Christopher Daly,Mark Sleeman,Gavin Thurston,Zhe LiPubMed 37058568
- Scientific reports 12:140792022Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Carbonaro,Jeffrey Lee,Evangelos Pefanis,Mathieu Desclaux,Kehui Wang,Alexander Pennington,Hui Huang,Alejo Mujica,Jose Rojas,Roxanne Ally,Daniel Kennedy,Michael Brown,Vitaliy Rogulin,Sven Moller-Tank,Leah Sabin,Brian Zambrowicz,Gavin Thurston,Zhe LiPubMed 35982097
- Frontiers in endocrinology 13:8953602022LncRNA MALAT1 Aggravates Renal Tubular Injury Activating LIN28A and the Nox4/AMPK/mTOR Signaling Axis in Diabetic Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanai Song,Yinyin Chen,Zhiwen Liu,Hong Liu,Li Xiao,Lin Sun,Jiali Wei,Liyu HePubMed 35813614
- ALTEX 40:103-1162022Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark C Daley,Ulrike Mende,Bum-Rak Choi,Patrick D McMullen,Kareen L K CoulombePubMed 35648122
- ALTEX 40:34–522022Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessmentApplications:Unspecified applicationReactive species:Unspecified reactive speciesAlex N Cayley,Robert S Foster,Emma Hill,Steven Kane,Grace Kocks,Alun Myden,Daniel Newman,Susanne A Stalford,Jonathan D Vessey,Reza Zarei,Antonio Anax F De OliveiraPubMed 35575642
- ALTEX 40:61-822022A microfluidic thyroid-liver platform to assess chemical safety in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Kühnlenz,Diana Karwelat,Thomas Steger-Hartmann,Marian Raschke,Sophie Bauer,Özlem Vural,Uwe Marx,Helen Tinwell,Remi BarsPubMed 35536601
- Biomedical reports 16:432022Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Li,Yang Zhao,Jeffrey J Yourick,Robert L Sprando,Xiugong GaoPubMed 35371477
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:1335-13462022Chronic cerebral hypoperfusion and blood-brain barrier disruption in uninjured brain areas of rhesus monkeys subjected to transient ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingqian Zhang,Bangcheng Zhao,Qi Lai,Qinxi Li,Xun Tang,Yinbing Zhang,Zhixiang Pan,Qiang Gao,Zhihui ZhongPubMed 35137610
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.125 - 200 ng/mL
- Assay time
- 3h
- Sensitivity
- = 0.79 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
What's included?
Recommended products
Human Albumin ELISA kit is a sandwich ELISA used to quantify human albumin (ALB) with a sensitivity of 0.79 ng/mL
- Broad sample compatibility – works with urine, saliva, milk, cerebrospinal fluid (CSF), cell culture supernatants, cell lysates, and tissue samples
- Wide dynamic range – Detects albumin levels from 3.125–200 ng/mL
- Efficient assay process – Total assay time of approximately 3 hours
- Comprehensive kit components: Includes pre-coated 96-well microplate
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.125 - 200 ng/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- = 0.79 ng/mL
- General recovery
- 99%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human Albumin ELISA Kit ab108788 is a sandwich ELISA to measure Human Albumin (ALB) in Tissue, Cerebral Spinal Fluid (CSF), Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate with a sensitivity of 0.79 ng/mL.
How the assay works
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Human Albumin ELISA Kit ab108788 protocol summary
1. Add samples or standards to the wells
2. Incubate for 1 hour then wash
3. Add biotinylated detector antibody
4. Incubate for 30 min then wash
5. Add the SP-conjugate
6. Incubate for 30 min then wash
7. Add the chromogen substrate
8. Incubate for 25 min
9. Add stop solution and read at 450 nm
How other researchers are using Human Albumin ELISA kit ab108788
The Human Albumin ELISA kit ab108788 has been used in publications to assess therapeutic effects of adipose-derived stem cells on acute liver failure (1), effects of GP130 signaling in liver stenosis(2), and potential therapeutic targets for diabetic nephropathy (3).
References:
(1)Xiaoyan Zhang et al. 2023, PMID: 37078751,
(2)Zhe Li et al. 2023 PMID: 37058568,
(3)Liyu He et al. 2022, PMID: 35813614
Get results in 90 minutes our SimpleStep ELISA® kit:
- Human Albumin ELISA Kit Human Albumin ELISA Kit ab179887
Related and recommended products
Human Albumin ELISA kit ab108788 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Human ALT ELISA kit Human ALT ELISA Kit ab234578 and Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
These ELISA kits are also available for Mouse/Rat:
- Mouse Albumin ELISA kit Mouse Albumin ELISA Kit ab207620
- Mouse ALT ELISA kit Mouse ALT ELISA Kit ab282882
- Mouse AST ELISA kit Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882
- Rat Albumin ELISA kit Rat Albumin ELISA Kit ab235642
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit Rat AST ELISA Kit (Aspartate Aminotransferase) ab263883
Kidney function marker assays include:
- Creatinine Assay Kit Creatinine Assay Kit ab65340 – suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric) Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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3 product images
ELISA - Human Albumin ELISA Kit (ab108788)
Standard curve with background signal subtracted (duplicates; +/- SD).
ELISA - Human Albumin ELISA Kit (ab108788)
Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).
Sandwich ELISA - Human Albumin ELISA Kit (ab108788)
Representative Standard Curve using ab108788
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