Human Albumin ELISA Kit

Product details

Human Albumin ELISA Kit ab108788 is a sandwich ELISA to measure Human Albumin (ALB) in Tissue, Cerebral Spinal Fluid (CSF), Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate with a sensitivity of 0.79 ng/mL.

How the assay works

An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.

Human Albumin ELISA Kit ab108788 protocol summary

1. Add samples or standards to the wells
2. Incubate for 1 hour then wash
3. Add biotinylated detector antibody
4. Incubate for 30 min then wash
5. Add the SP-conjugate
6. Incubate for 30 min then wash
7. Add the chromogen substrate
8. Incubate for 25 min
9. Add stop solution and read at 450 nm

How other researchers are using Human Albumin ELISA kit ab108788

The Human Albumin ELISA kit ab108788 has been used in publications to assess therapeutic effects of adipose-derived stem cells on acute liver failure ⁽¹⁾, effects of GP130 signaling in liver stenosis⁽²⁾, and potential therapeutic targets for diabetic nephropathy ⁽³⁾.

References:

⁽¹⁾Xiaoyan Zhang et al. 2023, PMID: 37078751,
⁽²⁾Zhe Li et al. 2023 PMID: 37058568,
⁽³⁾Liyu He et al. 2022, PMID: 35813614

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- Human Albumin ELISA Kit ab179887

Related and recommended products

Human Albumin ELISA kit ab108788 is commonly used by researchers to measure liver or kidney function.

Liver function ELISA assays include Human ALT ELISA kit ab234578 and Human AST ELISA Kit ab263881

These ELISA kits are also available for Mouse/Rat:

- Mouse Albumin ELISA kit ab207620
- Mouse ALT ELISA kit ab282882
- Mouse AST ELISA kit ab263882
- Rat Albumin ELISA kit ab235642
- Rat ALT ELISA kit ab234579
- Rat AST ELISA kit ab263883

Kidney function marker assays include:

- Creatinine Assay Kit ab65340 – suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.