Human Albumin ELISA Kit ab179887 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 0.1 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Cynomolgus monkey, Human
0.7 - 45 ng/mL
1h 30m
= 0.1 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, PRO2044, PRO1708, PRO0903, PRO2619, PRO2675, UNQ696/PRO1341, GIG42, GIG20, ALB, Albumin
Human Albumin ELISA Kit ab179887 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 0.1 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Cynomolgus monkey, Human
0.7 - 45 ng/mL
1h 30m
Microplate
= 0.1 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.2 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 104 - 105 % |
Sample type Serum | Average % = 100 | Range 92 - 110 % |
Sample type EDTA Plasma | Average % = 104 | Range 97 - 109 % |
Sample type Heparin Plasma | Average % = 98 | Range 89 - 105 % |
Sample type Citrate plasma | Average % = 101 | Range 100 - 103 % |
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Human Albumin ELISA kit ab179887 is a rapid single-wash 90-min ELISA kit to measure Human Albumin in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 0.1 ng/mL.
How the assay works
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Human Albumin ELISA kit ab179887 protocol summary
Flexible assay format
For more flexibility we offer the antibody pair used in this kit in a carrier-free format, ready for conjugation:
Assay Specificity
This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.
Species Reactivity
This kit recognizes human Albumin and Cynomolgus monkey albumin protein. If you require a kit that is human-specific, please see Human Albumin ELISA Kit ab227933.
Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
How other researchers are using Human Albumin ELISA Kit ab179887
Human Albumin ELISA kit ab179887 has been used in publications for the assessment of liver function in the construction of liver organoids or spheroids1,2 or for the assessment of Drug Induced Liver Toxicity (DILI)3,4
References: 1-Shuo Bai et al. 2023, PMID: 37199010, 2-Taniguchi H et al. 2023 PMID: 37108241, 3-Levner D et al. 2022, PMID: 36473994, 4-Ronxhi et al. 2022, PMID: 35790095.
Related and recommended products
Human Albumin ELISA kit ab179887 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Human ALT ELISA kit Human ALT ELISA Kit ab234578 and Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881.
These ELISA kits are also available for Mouse/Rat:
Kidney function marker assays include:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin often referred to as serum albumin is a major protein in the bloodstream known for its role in maintaining osmotic pressure and transporting various molecules. It has a molecular mass of approximately 66.5 kDa. Synthesized in the liver albumin can be found throughout the body in the circulatory system. Besides its primary location in human plasma variants such as mouse serum albumin can be studied in animal models providing insight into albumin's function across species.
Albumin carries out essential tasks like transporting substances such as hormones vitamins and drugs due to its high binding capacity. It serves as a vital protein for maintaining blood pH balance. Albumin does not function as part of a larger protein complex but acts independently to bind and transport various ligands thereby playing a role in removing free radicals and offering antioxidative protection.
The transport and regulatory functions of albumin integrate into key biological systems like the Renin-Angiotensin System and drug metabolism pathways. Within these pathways albumin interacts with enzymes and other proteins facilitating drug distribution and metabolism. Its binding abilities allow it to modulate the bioavailability of active compounds acting in concert with proteins like globulins that also play roles in transporting biomolecules.
Low levels of albumin a condition known as hypoalbuminemia associate with liver diseases such as cirrhosis and chronic kidney disease impacting the body's ability to maintain fluid and electrolyte balance. Albumin's interaction with proteins like globulin can alter in these conditions affecting protein binding capacity and exacerbating disease symptoms. Tools like the albumin ELISA kit and albumin BCG assay facilitate the measurement of albumin levels in clinical diagnostics and research aiding in disease detection and monitoring.
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Human Albumin standard curve comparison.
Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.
The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.
Linearity of dilution for spiked albumin.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution for albumin.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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