Skip to main content

Human Albumin ELISA Kit ab179887 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 0.1 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation


Images

Sandwich ELISA - Human Albumin ELISA Kit (AB179887), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB179887), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB179887), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB179887), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB179887), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Cynomolgus monkey, Human

Range

0.7 - 45 ng/mL

Assay time

1h 30m

Sensitivity

= 0.1 ng/mL

Abcam Recommends

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

What's included?

1 x 384 Tests
Components
10X Human Albumin Capture Antibody
1 x 600 µL
10X Human Albumin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
2 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 4BI
1 x 6 mL
Native Human Serum Albumin protein (Lyophilized) (ab205808)
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 250 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human Albumin ELISA Kit ab179887 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 0.1 ng/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Cynomolgus monkey, Human

Range

0.7 - 45 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 0.1 ng/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

5.2

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

7.6

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 104

Range

104 - 105 %

Sample type

Serum

Average %

= 100

Range

92 - 110 %

Sample type

EDTA Plasma

Average %

= 104

Range

97 - 109 %

Sample type

Heparin Plasma

Average %

= 98

Range

89 - 105 %

Sample type

Citrate plasma

Average %

= 101

Range

100 - 103 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Albumin ELISA kit ab179887 is a rapid single-wash 90-min ELISA kit to measure Human Albumin in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 0.1 ng/mL.

How the assay works
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Human Albumin ELISA kit ab179887 protocol summary

  • 1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hour at room temperature.
  • 2. Wash
  • 3. Add TMB development solution - incubate for 10 min
  • 4. Add Stop solution
  • 5. Read the results on a plate reader at 450 nm

Flexible assay format
For more flexibility we offer the antibody pair used in this kit in a carrier-free format, ready for conjugation:

Assay Specificity
This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.

Species Reactivity
This kit recognizes human Albumin and Cynomolgus monkey albumin protein. If you require a kit that is human-specific, please see Human Albumin ELISA Kit ab227933.

Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.

How other researchers are using Human Albumin ELISA Kit ab179887
Human Albumin ELISA kit ab179887 has been used in publications for the assessment of liver function in the construction of liver organoids or spheroids1,2 or for the assessment of Drug Induced Liver Toxicity (DILI)3,4

References: 1-Shuo Bai et al. 2023, PMID: 37199010, 2-Taniguchi H et al. 2023 PMID: 37108241, 3-Levner D et al. 2022, PMID: 36473994, 4-Ronxhi et al. 2022, PMID: 35790095.

Related and recommended products
Human Albumin ELISA kit ab179887 is commonly used by researchers to measure liver or kidney function.

Liver function ELISA assays include Human ALT ELISA kit Human ALT ELISA Kit ab234578 and Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881.

These ELISA kits are also available for Mouse/Rat:

Kidney function marker assays include:

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Albumin often referred to as serum albumin is a major protein in the bloodstream known for its role in maintaining osmotic pressure and transporting various molecules. It has a molecular mass of approximately 66.5 kDa. Synthesized in the liver albumin can be found throughout the body in the circulatory system. Besides its primary location in human plasma variants such as mouse serum albumin can be studied in animal models providing insight into albumin's function across species.

Biological function summary

Albumin carries out essential tasks like transporting substances such as hormones vitamins and drugs due to its high binding capacity. It serves as a vital protein for maintaining blood pH balance. Albumin does not function as part of a larger protein complex but acts independently to bind and transport various ligands thereby playing a role in removing free radicals and offering antioxidative protection.

Pathways

The transport and regulatory functions of albumin integrate into key biological systems like the Renin-Angiotensin System and drug metabolism pathways. Within these pathways albumin interacts with enzymes and other proteins facilitating drug distribution and metabolism. Its binding abilities allow it to modulate the bioavailability of active compounds acting in concert with proteins like globulins that also play roles in transporting biomolecules.

Associated diseases and disorders

Low levels of albumin a condition known as hypoalbuminemia associate with liver diseases such as cirrhosis and chronic kidney disease impacting the body's ability to maintain fluid and electrolyte balance. Albumin's interaction with proteins like globulin can alter in these conditions affecting protein binding capacity and exacerbating disease symptoms. Tools like the albumin ELISA kit and albumin BCG assay facilitate the measurement of albumin levels in clinical diagnostics and research aiding in disease detection and monitoring.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Human Albumin standard curve comparison.

    Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.

    The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Serum from eight individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Linearity of dilution for spiked albumin.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Linearity of dilution for albumin.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com