AB227933

Human Albumin ELISA Kit

Name: Human Albumin ELISA Kit (ab227933) | Abcam
Brand: Abcam Limited
SKU: AB227933
Price: 820 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(2 Publications)

Human Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Albumin ELISA kit with a sensitivity of 30 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

7 Images

ELISA - Human Albumin ELISA Kit (AB227933)

ELISA

Supplier Data

ELISA - Human Albumin ELISA Kit (AB227933)

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Interpolated concentrations of native Albumin in human serum and plasma samples.

The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 5 million, plasma (citrate) 1 : 5 million, plasma (heparin) 1 : 5 million, and plasma (EDTA) 1 : 10 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 39.3 mg/mL in serum, 34.4 mg/mL in plasma (citrate), 30.8 mg/mL in plasma (heparin), and 34.5 mg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Example of human Albumin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 33.31 mg/mL with a range of 22.5 – 46.2 mg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Albumin ELISA Kit (AB227933)

Interpolated concentrations of native Albumin in human urine samples.

The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6,290 ng/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 30 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

You may be interested in:

AB244051

Human Albumin Antibody Pair - BSA and Azide free

0 Reviews

View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style

left-column

Product details

Human Albumin ELISA Kit ab227933 is a rapid single-wash 90-min ELISA kit to measure Human Albumin in urine, serum and plasma. This SimpleStep ELISA® sensitivity is 30 pg/mL.

How the assay works

Human Albumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and purified Human IgA protein in serum, plasma, milk, urine, saliva, and cell culture media samples only. Cell and tissue extracts have not been tested with this kit.

Human Albumin ELISA Kit ab227933 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples

Our 384-well Human Albumin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- ab244051
- ab244696
- ab244962

Related and recommended products

Human Albumin ELISA kit ab227933 is commonly used by researchers to measure liver or kidney function.

Liver function ELISA assays include Human ALT ELISA kit ab234578 and Human AST ELISA Kit ab263881

These ELISA kits are also available for Mouse/Rat:

- Mouse Albumin ELISA kit ab207620
- Mouse ALT ELISA kit ab282882
- Mouse AST ELISA kit ab263882
- Rat Albumin ELISA kit ab235642
- Rat ALT ELISA kit ab234579
- Rat AST ELISA kit ab263883

Kidney function marker assays include:

- Creatinine Assay Kit ab65340 – suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "106 - 133 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "108 - 121 %", "average": "= 115" }, { "sample": "Urine", "range": "101 - 107 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "104 - 116 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "106 - 117 %", "average": "= 112" } ]

style

left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab227933-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB227933-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB227933-CMP11" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227933-CMP10" }, { "size":"2 x 1 Vial", "name":"Native Human Serum Albumin protein (Lyophilized) (ab205808)", "number":"AB227933-CMP08" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227933-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Capture Antibody", "number":"AB227933-CMP06" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Detector Antibody", "number":"AB227933-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB227933-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227933-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB227933-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227933-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227933-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB227933-CMP11" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227933-CMP10" }, { "size":"2 x 1 Vial", "name":"Native Human Serum Albumin protein (Lyophilized) (ab205808)", "number":"AB227933-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Capture Antibody", "number":"AB227933-CMP06" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227933-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Detector Antibody", "number":"AB227933-CMP07" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227933-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227933-CMP09" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227933-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227933-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227933-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Native Human Serum Albumin protein (Lyophilized) (ab205808)", "number":"AB227933-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP", "number":"AB227933-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Capture Antibody", "number":"AB227933-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227933-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227933-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Detector Antibody", "number":"AB227933-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227933-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227933-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227933-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

style

left-column

Product protocols

style

left-column

Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information ALB

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of pharmaceutics 609:121076 PubMed34481886

2021

Gemcitabine lipid prodrug nanoparticles: Switching the lipid moiety and changing the fate in the bloodstream.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonore Coppens,Didier Desmaële,Timothée Naret,Sébastien Garcia-Argote,Sophie Feuillastre,Grégory Pieters,Catherine Cailleau,Jean-Louis Paul,Bastien Prost,Audrey Solgadi,Jean-Philippe Michel,Magali Noiray,Patrick Couvreur,Simona Mura

BMC nephrology 21:2 PubMed31892319

2019

Removal of large middle molecules via haemodialysis with medium cut-off membranes at lower blood flow rates: an observational prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Hoon Kim,Seok-Hyung Kim,Tae Yeon Kim,Hae Yeul Park,Kwon Soo Jung,Moon Hyoung Lee,Jong Hyun Jhee,Jung Eun Lee,Hoon Young Choi,Hyeong Cheon Park

View all publications

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

websiteProtocolBooklet

style

right-column

Associated Products

Select an associated product type

Alternative Product

Assay Kits

AB108787

Human Albumin ELISA Kit

elisa-kits

human-albumin-elisa-kit-ab108787

(0 reviews)

Alternative Product

Assay Kits

AB108788

Human Albumin ELISA Kit

elisa-kits

human-albumin-elisa-kit-ab108788

(2 reviews)

Alternative Product

Assay Kits

AB229386

Human Albumin ELISA Kit, Fluorescent

elisa-kits

human-albumin-elisa-kit-fluorescent-ab229386

(0 reviews)

Alternative Product

Assay Kits

AB179887

Human Albumin ELISA Kit

elisa-kits

human-albumin-elisa-kit-ab179887

(1 reviews)

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com