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Human Albumin ELISA Kit ab227933 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 30 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput


Images

Sandwich ELISA - Human Albumin ELISA Kit (AB227933), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB227933), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB227933), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB227933), expandable thumbnail
  • Sandwich ELISA - Human Albumin ELISA Kit (AB227933), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Sensitivity

= 30 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

What's included?

1 x 384 Tests
Components
10X Human Albumin Capture Antibody
1 x 600 µL
10X Human Albumin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
2 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent CP2
1 x 6 mL
Native Human Serum Albumin protein (Lyophilized) (ab205808)
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 250 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human Albumin ELISA Kit ab227933 is a single-wash 90-min SimpleStep ELISA used to quantify Human Albumin with a sensitivity of 30 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human Albumin sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 30 pg/mL

Precision

Intra assay

Sample

Serum

n

9

mean

-

SD

-

C.V.

4

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

12.6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 117

Range

106 - 133 %

Sample type

EDTA Plasma

Average %

= 115

Range

108 - 121 %

Sample type

Urine

Average %

= 104

Range

101 - 107 %

Sample type

Heparin Plasma

Average %

= 111

Range

104 - 116 %

Sample type

Citrate plasma

Average %

= 112

Range

106 - 117 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Albumin ELISA Kit (ab227933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Albumin with 30 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CROSS REACTIVITY Purified mouse, bovine, and cynomolgus monkey serum albumin were prepared at 1,000,1000, and 500 ng/mL, respectively, and assayed for cross reactivity. No cross-reactivity was observed.



SPECIES REACTIVITY This kit recognizes Human Albumin protein. Other species reactivity was determined by measuring 1:5 million serum or plasma samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse, Rat, Cow and Rhesus Macaque

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Associated diseases and disorders

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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6 product images

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Example of human Albumin standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Interpolated concentrations of native Albumin in human serum and plasma samples.

    The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:5 million, plasma (citrate) 1:5 million, plasma (heparin) 1:5 million, and plasma (EDTA) 1:10 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 39.3 mg/mL in serum, 34.4 mg/mL in plasma (citrate), 30.8 mg/mL in plasma (heparin), and 34.5 mg/mL in plasma (EDTA).

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Interpolated concentrations of native Albumin in human urine samples.

    The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6,290 ng/mL in urine.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 33.31 mg/mL with a range of 22.5 – 46.2 mg/mL.

  • Sandwich ELISA - Human Albumin ELISA Kit (ab227933), expandable thumbnail

    Sandwich ELISA - Human Albumin ELISA Kit (ab227933)

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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