JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325369

Human Albumin ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Human Albumin ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human Albumin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :0.045 - 100 pg/mL with a sensitivity of 5 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

5 Images
Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)
  • sELISA

Lab

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)
  • sELISA

Lab

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)
  • sELISA

Lab

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)

Dynamic range comparison between the Chemiluminescent Human Albumin SimpleStep ELISA, colorimetric Human Albumin SimpleStep ELISA (ab179887). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)
  • sELISA

Lab

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)

The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000,000, plasma (EDTA) 1 : 4,000,000, plasma (citrate) 1 : 8,000,000, and plasma (heparin) 1 : 8,000,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)
  • sELISA

Lab

Sandwich ELISA - Human Albumin ELISA Kit, Chemiluminescent (AB325369)

Standard curve comparison between the Chemiluminescent Human Albumin SimpleStep ELISA and colorimetric Human Albumin SimpleStep ELISA (ab179887) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5 pg/mL

Range

0.0457 - 100 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Albumin ELISA Kit, Chemiluminescent (ab325369) is a rapid single-wash sandwich 55-min ELISA to measure Human Albumin in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Media with a sensitivity of 5 pg/ml.

How the assay works

Human Albumin ELISA Kit, Chemiluminescent (ab325369) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human Albumin (ab325369) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR20195-105] to Human Serum Albumin - BSA and Azide free (Capture) ab244696

- Rabbit monoclonal [EPR20195-16] to Human Serum Albumin - BSA and Azide free (Detector) ab244962

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "92 - 110 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "100 - 103 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "97 - 109 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "89 - 105 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "104 - 105 %", "average": "= 104" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325369-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325369-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325369-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Albumin Lyophilized Purified Protein", "number":"AB325369-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325369-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325369-CMP08", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325369-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325369-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Detector Antibody", "number":"AB325369-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Albumin Capture Antibody", "number":"AB325369-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325369-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com