AB270205

Human ALCAM ELISA Kit (CD166)

Recombinant
SimpleStep
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Human ALCAM ELISA Kit (CD166) is a single-wash 90-min Simplestep used to quantify Human ALCAM (CD166) with a sensitivity of 0.02 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule

4 Images

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

sELISA

Supplier Data

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Interpolated concentrations of native ALCAM in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).

The concentrations of ALCAM were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 3.75%, plasma (EDTA) 2.5%, plasma (citrate) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 94.3 ng/mL in serum, 73.1 ng/mL in plasma (EDTA), 112.0 ng/mL in plasma (citrate), and 82.9 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Example of human ALCAM standard curve in Sample Diluent NS.

The ALCAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Interpolated concentrations of native ALCAM in human HT-1080 cell extract based on a 250 μg/mL extract load.

The concentrations of ALCAM were measured in duplicate and interpolated from the ALCAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Serum from eight individual healthy ALCAM gender donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALCAM concentration was determined to be 111 ng/mL with a range of 86 – 133 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.02 ng/mL

Range

0.188 - 12 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ALCAM ELISA Kit (CD166) (ab270205) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALCAM (CD166) protein in cell culture media, cell lysate, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALCAM (CD166) with 0.02 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a protein encoded by the *ALCAM* gene in humans. ALCAM is a type I transmembrane glycosylated member of the immunoglobulin superfamily of proteins and is mainly involved in the processes of cell adhesion and migration. This protein forms a homo-dimer through the extracellular domain portion or can form a hetero-dimer with T-cell differentiation antigen CD6 with the same region of ALCAM, and the affinity for CD6 is higher than self-association. Interaction with CD6 mediates promotes T-cell activation and proliferation, as well as the formation and maturation of the immunological synapse. ALCAM also mediates attachment of dendritic cells onto endothelial cells by homotypic interaction.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Serum", "range": "114 - 123 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "104 - 125 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "101 - 102 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "111 - 116 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "108 - 125 %", "average": "= 118" }, { "sample": "Cell Lysate", "range": "95 - 108 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALCAM also known as Activated Leukocyte Cell Adhesion Molecule is a protein with a molecular weight of roughly 65-105 kDa due to post-translational modifications. ALCAM expresses in various tissues including the nervous system immune system and endothelial cells. It functions as a cell adhesion molecule and facilitates cell-cell interactions by binding to its ligands like itself and CD6. This interaction plays an important role for maintaining tissue architecture and cell migration.

Biological function summary

The interactions that ALCAM supports are important for processes such as synapse formation and stabilization in the nervous system. ALCAM does not act alone; it participates in complexes with other cell adhesion molecules enhancing signals for tissue development and repair. ALCAM's expression patterns reveal its involvement in leukocyte trafficking and immune responses indicating its importance in maintaining physiological homeostasis.

Pathways

ALCAM plays roles in pathways like the MAPK and PI3K/Akt signaling pathways which are important for cell survival and proliferation. Through these ALCAM interacts with proteins such as ERK1/2 and AKT. These pathways influence cellular responses to external stimuli highlighting the protein's role in growth and differentiation processes. ALCAM's participation in these pathways links it with cellular responses to stress and growth signals.

ALCAM shows associations with cancer metastasis and autoimmune diseases. In cancer changes in ALCAM expression affect tumor progression and metastasis possibly involving interactions with other adhesion molecules and integrins. In autoimmune disorders like multiple sclerosis ALCAM may influence disease progression through interactions with lymphocyte markers. Understanding these connections helps in developing potential therapeutic strategies to modulate ALCAM's function in pathological states.

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Product protocols

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Target data

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed : 15048703, PubMed : 15496415, PubMed : 16352806, PubMed : 23169771, PubMed : 24945728, PubMed : 7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed : 15048703, PubMed : 16352806, PubMed : 24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed : 15294938, PubMed : 16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed : 15496415, PubMed : 16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed : 29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed : 24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed : 23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed : 15496415, PubMed : 23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).. Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

See full target information ALCAM

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human ALCAM ELISA Kit (CD166)

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (AB270205)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise