AB214024

Human ALDH1A1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human ALDH1A1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ALDH1A1 with a sensitivity of 220 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1

5 Images

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

sELISA

Supplier Data

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Example of human ALDH1A1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Example of human ALDH1A1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Interpolated concentrations of native ALDH1A1 in human A549, Hep G2, fetal brain, and liver homogenate extract samples.

Based on 25 μg/mL, 20 μg/mL, 100 μg/mL, and 1.25 μg/mL extract loads, respectively. The concentrations of ALDH1A1 were measured in duplicate and interpolated from the ALDH1A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 59.4 ng/mL, 76.8 ng/mL, 13.1 ng/mL, and 10.5 ng/mL in A549, Hep G2, fetal brain, and liver homogenate extract samples, respectively.

sELISA

Supplier Data

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 18.98 ng/mL with a range of 1.72 – 143.05 ng/mL.

sELISA

Unknown

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Interpolated concentrations of native ALDH1A1 in human serum and plasma samples.

The concentrations of ALDH1A1 were measured in duplicates, interpolated from the ALDH1A1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 4.38 ng/ml in serum, 7.37 ng/ml in plasma (citrate), 11.12 ng/ml in plasma (heparin), and 14.40 ng/ml in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 220 pg/mL

Range

1.25 - 80 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ALDH1A1 ELISA Kit (ab214024) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALDH1A1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALDH1A1 with 220 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ALDH1A1 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase follows alcohol dehydrogenase in the major pathway of alcohol metabolism. Notably, there are two major aldehyde dehydrogenase isozymes in the liver: cytosolic and mitochondrial. This gene encodes the cytosolic isozyme. Finally, human ALDH1A1 is 501 amino acids in length. Mouse and rat ALDH1A1 are 87% and 86% identical to human ALDH1A1, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "A549 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "A549 Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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Recovery

[ { "sample": "Serum", "range": "73 - 83 %", "average": "= 76" }, { "sample": "EDTA Plasma", "range": "78 - 96 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "109 - 110 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "70 - 80 %", "average": "= 75" }, { "sample": "Citrate plasma", "range": "68 - 80 %", "average": "= 74" }, { "sample": "Cell culture media", "range": "105 - 110 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

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Product protocols

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Target data

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed : 12941160, PubMed : 15623782, PubMed : 17175089, PubMed : 19296407, PubMed : 25450233, PubMed : 26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed : 12941160, PubMed : 15623782, PubMed : 19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed : 17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

See full target information ALDH1A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6318 PubMed36274088

2022

A noncanonical function of EIF4E limits ALDH1B1 activity and increases susceptibility to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chen,Jun Huang,Chunhua Yu,Jiao Liu,Wanli Gao,Jingbo Li,Xinxin Song,Zhuan Zhou,Changfeng Li,Yangchun Xie,Guido Kroemer,Jinbao Liu,Daolin Tang,Rui Kang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human ALDH1A1 ELISA Kit

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Sandwich ELISA - Human ALDH1A1 ELISA Kit (AB214024)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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