JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313505

Human ALDH4A1/P5CDH ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human ALDH4A1/P5CDH ELISA Kit is a Sandwich ELISA for the measurement of Human ALDH4A1/P5CDH in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ALDH4, P5CDH, ALDH4A1, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase

1 Images
Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (AB313505)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (AB313505)

Example of standard curve using ab313505. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.48 ng/mL

Range

0.48 - 120 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB185208

Anti-ALDH4A1/P5CDH antibody [EPR14287]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH4A1/P5CDH antibody [EPR14287] (AB185208)

  • 0
  • 0
Proteins & Peptides

AB160138

Recombinant Human ALDH4A1/P5CDH protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ALDH4A1/P5CDH protein (GST tag N-Terminus) (AB160138)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human ALDH4A1/P5CDH ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALDH4A1/P5CDH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ALDH4A1/P5CDH coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Cell culture media", "range": "123 - 136 %", "average": "= 131" }, { "sample": "Plasma", "range": "84 - 141 %", "average": "= 100.7" }, { "sample": "Serum", "range": "100 - 104 %", "average": "= 101.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313505-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human ALDH4A1/P5CDH Detection Antibody", "number":"AB313505-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313505-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313505-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313505-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ALDH4A1/P5CDH Antibody-coated ELISA Plate", "number":"AB313505-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ALDH4A1/P5CDH Protein Standard", "number":"AB313505-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313505-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB313505-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.
Biological function summary

ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.

Pathways

ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.

Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.
style
left-column

Product protocols

style
left-column

Target data

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
See full target information ALDH4A1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com