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Human ALDH4A1/P5CDH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALDH4A1/P5CDH in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (AB313505), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.48 - 120 ng/mL
Sensitivity
= 0.48 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
400X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human ALDH4A1/P5CDH Detection Antibody
2 x 1 Vial
Human ALDH4A1/P5CDH Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human ALDH4A1/P5CDH Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human ALDH4A1/P5CDH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALDH4A1/P5CDH in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.48 - 120 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.48 ng/mL

Recovery

Sample specific recovery

Sample type
Cell culture media
Average %
= 131
Range
123 - 136 %
Sample type
Plasma
Average %
= 100.7
Range
84 - 141 %
Sample type
Serum
Average %
= 101.1
Range
100 - 104 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human ALDH4A1/P5CDH ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALDH4A1/P5CDH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ALDH4A1/P5CDH coated on a 96-well plate.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.

Biological function summary

ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.

Pathways

ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.

Associated diseases and disorders

Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.

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Terms & Conditions.

1 product image

  • Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (ab313505), expandable thumbnail

    Sandwich ELISA - Human ALDH4A1/P5CDH ELISA Kit (ab313505)

    Example of standard curve using ab313505. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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