Human ALDH4A1/P5CDH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALDH4A1/P5CDH in Human in Cell culture supernatant, Plasma, Serum samples.
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
ALDH4, P5CDH, ALDH4A1, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase
Human ALDH4A1/P5CDH ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALDH4A1/P5CDH in Human in Cell culture supernatant, Plasma, Serum samples.
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture media | Average % = 131 | Range 123 - 136 % |
Sample type Plasma | Average % = 100.7 | Range 84 - 141 % |
Sample type Serum | Average % = 101.1 | Range 100 - 104 % |
Human ALDH4A1/P5CDH ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALDH4A1/P5CDH in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ALDH4A1/P5CDH coated on a 96-well plate.
ALDH4A1 also known as P5CDH is an enzyme responsible for catalyzing the conversion of pyrroline-5-carboxylate to glutamate in the mitochondria. It has a molecular mass of approximately 65 kDa and plays an essential role in the proline degradation pathway. The enzyme is primarily expressed in the liver kidney and muscle tissues where it supports the metabolism of amino acids and energy production.
ALDH4A1 is involved in the L-proline degradation pathway converting substrates to downstream metabolic intermediates. While it operates mainly as an individual enzyme it facilitates the integration of amino acid metabolism with cellular energy cycles. A deficiency in ALDH4A1 activity disrupts normal nitrogen balance and mitochondrial function illustrating its relevance to cellular homeostasis.
ALDH4A1 plays a significant role in the urea cycle and the proline degradation pathway. It interacts with enzymes like delta-1-pyrroline-5-carboxylate synthetase which provides substrates for its enzymatic activity. These interactions are important for maintaining nitrogen homeostasis and chemical energy balance within cells highlighting its contributions to metabolic circuits.
Mutations and deficiencies in ALDH4A1 lead to hyperprolinemia type II and can contribute to neurological issues. The protein interacts with other cellular components such as ALDH3A2 which also participates in detoxifying reactive aldehydes. Abnormal ALDH4A1 function can exacerbate proline accumulation and neurotoxic effects in affected individuals making it a significant focus for understanding metabolic disorders.
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Example of standard curve using ab313505. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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