AB325255

Human ALK-1 (ACVRL1) ELISA Kit

SimpleStep
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Human ALK-1 (ACVRL1) ELISA Kit is a Sandwich ELISA for the measurement of Human ALK-1 (ACVRL1) in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Interpolated concentration of native ALK-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : EA.hy926 cell extract 75 µg/mL and human lung tissue extract 125 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Example of human ALK-1 standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Interpolated concentration of native ALK-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 37.5 µg/mL EA.hy926 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Example of human ALK-1 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.65 pg/mL

Range

17.188 - 1100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ALK-1 (ACVRL1) ELISA Kit (ab325255) is a rapid single-wash sandwich 90-min ELISA to measure Human ALK-1 (ACVRL1) in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 9.65 pg/ml.

How the assay works

Human ALK-1 (ACVRL1) ELISA Kit (ab325255) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ALK-1 (ACVRL1) (ab325255) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human ALK-1 (ACVRL1) ELISA Kit only requires 12.5µL sample.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision & recovery information

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" } ]

Recovery

[ { "sample": "Cell Lysate", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "100 - 108 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

See full target information ACVRL1

Alternative Names

ACVRLK1, ALK1, ACVRL1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ALK-1 (ACVRL1) ELISA Kit

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision & recovery information

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Product promise