Human ALK-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALK-1 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.18 - 40 ng/mL
- Sensitivity
- = 0.18 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Alternative names
ACVRLK1, ALK1, ACVRL1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I
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Human ALK-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ALK-1 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.18 - 40 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.18 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 84.18 | Range 75 - 90 % |
Sample type Plasma | Average % = 74.81 | Range 72 - 78 % |
Sample type Cell culture media | Average % = 82.06 | Range 70 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human ALK-1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALK-1 in serum (human ALK-1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human ALK-1 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALK-1 also known as ACVRL1 or ALK1 protein is a receptor serine/threonine kinase with a molecular mass of approximately 55 kDa. It plays an important role in the TGF-beta superfamily signaling particularly in angiogenesis and blood vessel development. Expressed mainly in endothelial cells ALK-1 mediates cellular responses essential for maintaining vascular stability. The careful balance of its signaling is necessary indicating its importance in physiological conditions.
- Biological function summary
The function of ALK-1 extends to the formation and maintenance of vascular endothelium as part of the larger receptor complex. It interacts with ligands such as BMP9 and BMP10 which regulate endothelial cell proliferation and differentiation. ALK-1's role as part of this receptor complex highlights its involvement in controlling the fine balance between angiogenic and anti-angiogenic processes. Its biological activity is vital for healthy vascular development and homeostasis.
- Pathways
ALK-1 activation is a part of the BMP signaling pathway a branch of the larger TGF-beta pathway. This pathway is significant in various cellular processes including growth and differentiation. ALK-1 interacts with other signalling mediators like SMAD1/5/8 proteins which translocate to the nucleus to affect gene expression. Connecting with these pathways ALK-1 aligns closely with ACVR2A and ENG (Endoglin) which further highlight its role in vascular biology.
- Associated diseases and disorders
ALK-1 mutations or dysregulation leads to conditions such as hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH). These conditions showcase the important role ALK-1 plays in vascular integrity and signaling. ALK-1 is connected to disorders through interactions with proteins such as ENG further highlighting its involvement in vascular-related pathologies. Maintaining precise ALK-1 function is pivotal to preventing these disorders indicating its potential as a therapeutic target or biomarker.
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1 product image
Sandwich ELISA - Human ALK-1 ELISA Kit (ab314826)
Example of standard curve using ab314826. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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