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AB314826

Human ALK-1 ELISA Kit

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Human ALK-1 ELISA Kit is a Sandwich ELISA for the measurement of Human ALK-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

ACVRLK1, ALK1, ACVRL1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I

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Sandwich ELISA - Human ALK-1 ELISA Kit (AB314826)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALK-1 ELISA Kit (AB314826)

Example of standard curve using ab314826. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.18 ng/mL

Range

0.18 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB68703

Anti-ALK-1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody (AB68703)

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  • 1
Proteins & Peptides

AB70317

Recombinant human ALK-1 protein

Functional Studies - Recombinant human ALK-1 protein (AB70317)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ALK-1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ALK-1 in serum (human ALK-1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human ALK-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "75 - 90 %", "average": "= 84.18" }, { "sample": "Plasma", "range": "72 - 78 %", "average": "= 74.81" }, { "sample": "Cell culture media", "range": "70 - 107 %", "average": "= 82.06" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314826-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin", "number":"AB314826-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314826-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314826-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ALK-1 Detection Antibody", "number":"AB314826-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314826-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ALK-1 Antibody-coated ELISA Plate", "number":"AB314826-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314826-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ALK-1 Protein Standard", "number":"AB314826-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314826-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALK-1 also known as ACVRL1 or ALK1 protein is a receptor serine/threonine kinase with a molecular mass of approximately 55 kDa. It plays an important role in the TGF-beta superfamily signaling particularly in angiogenesis and blood vessel development. Expressed mainly in endothelial cells ALK-1 mediates cellular responses essential for maintaining vascular stability. The careful balance of its signaling is necessary indicating its importance in physiological conditions.
Biological function summary

The function of ALK-1 extends to the formation and maintenance of vascular endothelium as part of the larger receptor complex. It interacts with ligands such as BMP9 and BMP10 which regulate endothelial cell proliferation and differentiation. ALK-1's role as part of this receptor complex highlights its involvement in controlling the fine balance between angiogenic and anti-angiogenic processes. Its biological activity is vital for healthy vascular development and homeostasis.

Pathways

ALK-1 activation is a part of the BMP signaling pathway a branch of the larger TGF-beta pathway. This pathway is significant in various cellular processes including growth and differentiation. ALK-1 interacts with other signalling mediators like SMAD1/5/8 proteins which translocate to the nucleus to affect gene expression. Connecting with these pathways ALK-1 aligns closely with ACVR2A and ENG (Endoglin) which further highlight its role in vascular biology.

ALK-1 mutations or dysregulation leads to conditions such as hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH). These conditions showcase the important role ALK-1 plays in vascular integrity and signaling. ALK-1 is connected to disorders through interactions with proteins such as ENG further highlighting its involvement in vascular-related pathologies. Maintaining precise ALK-1 function is pivotal to preventing these disorders indicating its potential as a therapeutic target or biomarker.
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Product protocols

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Target data

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
See full target information ACVRL1
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websiteProtocolBooklet
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Product promise

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